Cryopreservation of mammalian oocytes with transmembrane-type protein

跨膜型蛋白冷冻保存哺乳动物卵母细胞

• 批准号: 22658085
• 负责人: KASHIWAZAKI Naomi
• 金额: $ 2.24万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658085/
• 关键词: 哺乳類; 卵; 凍結保存

The aim of the present study was to improve vitrification of mouse oocytes. We examined whether carboxyrated e-poly-L-lysine(COOH-PLL), a new cryoprotective agent, is available for vitrification of mouse embryos. When the oocytes were vitrified with 20%(v/v) ethylene glycol and 10%(w/v) COOH-PLL, the fertility and developmental ability were significantly higher than those in other groups. These embryos at the 2 cell stage were transferred to recipient females. Most of the recipients became pregnant and pups were delivered. In conclusion, our data clearly showed COOH-PLL is a useful cryoprotective agent for vitrification of mouse oocytes. These findings will contribute to improve oocyte vitrification in not only experimental animals but also clinical application for human infertility.

本研究旨在提高小鼠卵母细胞的玻璃化冷冻效果。我们研究了一种新的冷冻保护剂--羧基聚L赖氨酸(COOHPLL)是否可用于小鼠胚胎玻璃化冷冻。用20%(v/v)乙二醇组和10%(w/v)COOH-PLL组玻璃化冷冻，受精率和发育能力均显著高于其他组。这些2细胞期的胚胎被移植到受体雌性体内。大多数受试者都怀孕了，并产下了幼崽。综上所述，COOH-PLL对小鼠卵母细胞玻璃化冷冻是一种有效的冷冻保护剂。这些发现不仅将有助于改进卵母细胞玻璃化冷冻的实验动物，而且将有助于临床应用于人类不孕。

项目成果

Nuclear Replacement of In Vitro-Matured Porcine Oocytes by a Serial Centrifugation and Fusion Method

• DOI: 10.1111/j.1439-0531.2008.01324.x
• 发表时间: 2010-08-01
• 期刊: REPRODUCTION IN DOMESTIC ANIMALS
• 影响因子: 1.7
• 作者: Maedomari, N.;Kikuchi, K.;Kashiwazaki, N.
• 通讯作者: Kashiwazaki, N.

ガラス化保存したマウス前核期胚の再ガラス化保存後の生存性および発生能

玻璃化小鼠原核期胚胎再玻璃化保存后的活力和发育潜力

• 发表时间: 2011
• 作者: 柴尾友里恵;他
• 通讯作者: 他

Cryotop facilitates high developmental ability of re-vitrified mouse embryos

• 发表时间: 2011
• 作者: J. Ito;M. Kuramochi;A. Inoue;K. Yabe;Katsuyoshi Fujiwara;Osamu Nishikawa;K. Arishima;S. Sonoki;T. Masaoka;N. Kashiwazaki

Techniques for In Vitro and In Vivo Fertilization in the Rat

• DOI: 10.1007/978-1-60327-389-3_22
• 发表时间: 2010-01-01
• 期刊: RAT GENOMICS: METHODS AND PROTOCOLS
• 作者: Kashiwazaki, Naomi;Seita, Yasunari;Serikawa, Tadao
• 通讯作者: Serikawa, Tadao

Full-term development of rats from oocytes fertilized in vitro using cryopreserved ejaculated sperm

使用冷冻保存的射精精子进行体外受精卵母细胞的大鼠足月发育

• 期刊: Cryobiology
• 影响因子: 2.7
• 作者: Seita;et al.
• 通讯作者: et al.