Cryopreservation of mammalian oocytes with transmembrane-type protein

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The aim of the present study was to improve vitrification of mouse oocytes. We examined whether carboxyrated e-poly-L-lysine(COOH-PLL), a new cryoprotective agent, is available for vitrification of mouse embryos. When the oocytes were vitrified with 20%(v/v) ethylene glycol and 10%(w/v) COOH-PLL, the fertility and developmental ability were significantly higher than those in other groups. These embryos at the 2 cell stage were transferred to recipient females. Most of the recipients became pregnant and pups were delivered. In conclusion, our data clearly showed COOH-PLL is a useful cryoprotective agent for vitrification of mouse oocytes. These findings will contribute to improve oocyte vitrification in not only experimental animals but also clinical application for human infertility.