Development of evaluation methods for effects of environmental chemicals on the central nervous systems

环境化学物质对中枢神经系统影响评价方法的开发

• 批准号: 22659120
• 负责人: SAITO Takeshi
• 金额: $ 2.07万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659120/
• 关键词: 環境保健; 環境汚染化学物質; 中枢神経系; 影響評価法; アポトーシス; DNA切断; 分化; 衛生; 神経伝達物質

We developed evaluation methods for effects of environmental chemicals on the central nervous systems. First of all, the method for the quantification of DNA damage by chemicals in tissues and cultured cells was developed. Using the method, we evaluated apoptotic effects of diethyl phthalate parabens and a stevioside. Secondly, we developed an evaluation method for effects of chemicals on cell differentiation in PC12 cells. In addition, the evaluation method to detect apoptosis induced by metals and chelated metals was developed.

我们开发了评估环境化学物质对中枢神经系统影响的方法。首先，建立了组织和培养细胞中化学物质DNA损伤的定量方法。采用该方法，我们评估了邻苯二甲酸二乙酯对羟基苯甲酸酯和甜菊苷对细胞凋亡的影响。其次，我们建立了一种评价化学物质对PC12细胞分化影响的方法。此外，还建立了检测金属和螯合金属诱导细胞凋亡的评价方法。

项目成果

Effect ofquercetin intake during lactation on the AMPK activation in the hypothalamus of adult male rat offspring programmed by maternal protein restriction

哺乳期槲皮素摄入对母体蛋白质限制的成年雄性大鼠后代下丘脑 AMPK 激活的影响

• 发表时间: 2012
• 作者: Md. Tajuddin Sikder et al;Yongkun Sun et al;押谷仁;Masato Tanaka et al
• 通讯作者: Masato Tanaka et al

Effects of parabens on apoptosis induced by serum-free medium

对羟基苯甲酸酯对无血清培养基诱导细胞凋亡的影响

• DOI: 10.1080/03601234.2012.634341
• 发表时间: 2012
• 期刊: Journal of Environmental Science and Health
• 作者: Egawa M;Aoki K;Sun Y;Hosokawa T;Saito T and Kurasaki M
• 通讯作者: Saito T and Kurasaki M

Diethyl phthalate enhances apoptosis induced by serum deprivation inPC12 cells

邻苯二甲酸二乙酯增强 PC12 细胞血清剥夺诱导的细胞凋亡

• DOI: 10.1111/j.1742-7843.2012.00869.x
• 发表时间: 2012
• 期刊: Basic & Clinical Pharmacology& Toxicology
• 作者: Sun Y;Takahashi K;Saito T;Hosokawa T;Kurasaki M
• 通讯作者: Kurasaki M

Stevioside enhances apoptosis induced by serum deprivation in PC12 cells

甜菊苷增强 PC12 细胞血清剥夺诱导的细胞凋亡

• DOI: 10.3109/15376516.2012.658978
• 发表时间: 2012
• 期刊: Toxicology Mechanisms and Methods
• 影响因子: 3.2
• 作者: Takahashi K;Sun Y;Yanagiuchi I;Hosokawa T;Saito T;Komori M;Okino T and Kurasaki M
• 通讯作者: Okino T and Kurasaki M

Metal concentrationsof river water and sediments in West Java

西爪哇河水和沉积物的金属浓度

• DOI: 10.1007/s00128-011-0411-z
• 发表时间: 2011
• 期刊: Indonesia. Bulletin of Environmental Contamination and Toxicology
• 作者: Yasuda M;Yustiawati,Suhaemi M Syawal;Sikder;MT;Hosokawa T;Saito T.;Tanaka S. and Kurasaki M
• 通讯作者: Tanaka S. and Kurasaki M