Development of evaluation method of drug safety for children using cultured cells and tandem mass spectrometry

有些药物可能偶尔对儿童有毒，尽管对成年人无毒。我们使用体外探针酰卡尼汀(IVP)法，利用培养的成纤维细胞和串联质谱仪(MS/MS)来评估脂肪酸氧化(FAO)能力和药物的毒性。使用了来自粮农组织各种疾病(中长链疾病)的细胞，并获得了以下结果：阿司匹林(解热药)和丙戊酸(抗癫痫药物)可能导致粮农组织受损患者的细胞中粮农组织的一些抑制。苯扎贝特(降血脂药物)显著改善了粮农组织紊乱的细胞中的粮农组织。苯扎贝特可以成为粮农组织疾病的一种新的治疗选择。

There are some drugs which may occasionally toxic for children, despite non-toxic for adults. We use a method, in vitro probe acylcarnitine(IVP) assay, using cultured fibroblasts and tandem mass spectrometry(MS/MS) to evaluate fatty acid oxidation(FAO) capacity and toxicity of drugs. Cells from various FAO disorders(medium-to long-chain disorders) were used, and the following results were obtained : Aspirin(antipyretic), and valproic acid(anti-epileptic drug) potentially caused some inhibition of FAO in cells from impaired FAO patients. Bezafibrate(hypolipidemic drug) significantly improved FAO in cells from FAO disorders. Bezafibrate can be a new treatment option for FAO disorders.