Development of radiation-sensitizing anti-cancer agents using G2 checkpoint aberogator

使用 G2 检查点消除器开发放射增敏抗癌药物

• 批准号: 22700913
• 负责人: KOIZUMI Yukio
• 金额: $ 2.58万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700913/
• 关键词: 抗がん物質探索; ケミカルバイオロジー; 放射線; 細胞周期; シグナル伝達; 癌

The present study aims at examing the radiation-sensitizing effect of G2 checkpoint abrogators against solid cancers and discovering the new anti-cancer agent which brings both benefits, enhancing the therapeutic efficacy and decreasing adverse effects of cancer radiotherapy. We examined the effects of various G2 checkpoint abrogators on G2 arrest induction and antiproliferative effect by X-ray irradiation against esophageal cancer cell line, TE5. Cell cycle analysis and trypan blue exclusion assay showed that UCN-01 abrogated G2 arrest and potentiated growth inhibition, respectively. Furthermore, Western blotting demonstrated that UCN-01 decreased the protein level of Chk1 kinase activated by X-ray irradiation.

本研究旨在检测G2检查点去除剂对实体瘤的辐射增敏作用，寻找一种既能提高肿瘤放射治疗的疗效又能减少不良反应的新型抗癌剂。我们检测了不同的G2检查点去除剂对X射线照射对食管癌细胞系TE5的G2期阻滞诱导和抑制增殖作用的影响。细胞周期分析和台盼蓝拒染实验表明，UCN-01分别消除了G2期阻滞和增强了细胞生长抑制。此外，Western blotting显示UCN-01降低了X射线激活的Chk1激酶的蛋白水平。

项目成果

Synthesis and biological evaluation of malformin derivatives a fibrinolysis enhancer

纤溶促进剂马尔福明衍生物的合成及生物学评价

• 发表时间: 2012
• 作者: Koizumi Y;Nagai K;Hasumi K;Koyota S;Sugiyama T
• 通讯作者: Sugiyama T

Cosmc阻害による乳ガン細胞の増殖・転移抑制

通过 Cosmc 抑制抑制乳腺癌细胞增殖和转移

• 发表时间: 2010
• 作者: 明円悠;小代田宗一;馬莉;小泉幸央;杉山俊博
• 通讯作者: 杉山俊博

マウスエナメル芽細胞前駆細胞と象牙芽細胞前駆細胞の共培養による石灰化促進

通过小鼠成釉细胞祖细胞和成牙本质细胞祖细胞的共培养促进矿化

• 发表时间: 2010
• 作者: 小代田宗一;小代田理恵;堀川彩夏;牧野晃大;遊佐和之;小泉幸央;杉山俊博
• 通讯作者: 杉山俊博

Generation and characterization of intracellular single chain fragment variable (scFv) antibodies against ppGalNacTs

针对 ppGalNacTs 的细胞内单链片段可变 (scFv) 抗体的生成和表征

• 发表时间: 2010
• 作者: Ma L;Koyota S;Myoen Y;Koizumi Y;Sugiyama T
• 通讯作者: Sugiyama T

In vitro prominent bone regeneration by release zinc ion from Zn-modified implant.

• DOI: 10.1016/j.bbrc.2011.07.082
• 发表时间: 2011-08
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: K. Yusa;Osamu Yamamoto;M. Fukuda;S. Koyota;Yukio Koizumi;T. Sugiyama