Sialyllactose - modified 3-way junction DNA as a inhibitor of influenza hemagglutinin

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Sialyllactose(SL) is present on cellular surface and plays a important roles in the infection on influenza virus. The SL is recognized by hemagglutinin(HA) protein on the virus. A compound that inhibits the interaction between SL on the cell and hemagglutinin has been thought to prevent infection of influenza virus, because the amino acids sequence of sialic acid binding site in HAs are highly conserved among influenza virus strains. In this study, SL-modified dUTP was synthesized as a substrate of DNA polymerase, and these SLs were incorporated on 3-way junction DNAs. These DNAs have high affinity (15 nM) to the hemagglutinin on influenza virus A/PR/8/34(H1N1).These DNAs are expected to be used as influenza virus sensing or inhibitors of influenza infection.