Enhancement by liposome of semi-synthesis of specifically modified protein at the C-terminal and application to living cell

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In our previous study, semi-synthesis of protein specifically modified with lipo-philic compounds was carry out, but not effective. Denaturing protein during the specific condensation, purification of coupling product, and toxicity to live cell are to be adapted for improving effectively. To overcome these difficulties, liposome was employed in each step. As results, separation of coupling product from reaction mixture of condensation, and condensation avoiding denature of protein were carried out smoothly. However, use of liposome in coupling without detergent was not effective. Andmore, release of protein from the liposeme was not effective when liposeme horboring modified protein was applied to living cell.