What mechanisms determine the final destination of GPI-anchored proteins?

点击翻译按钮获取中文摘要

In fungi, a number of GPI-anchored proteins (GPI-APs) are retained to the plasma membrane, whereas others are transferred to the cell wall. We investigated amounts of GPI-AP in the cell wall and plasma membrane in Saccharomyces cerevisiae cwh43 deletion mutant cells, in which lipid moieties of GPI-APs are not converted to ceramide types. In cwh43 mutant cells, the amount of GPI-AP in the cell wall is increased, indicating that the conversion (lipid remodeling) to ceramides in GPI lipid moieties contributes to the retention of GPI-APs to the plasma membrane. In ted1 deletion mutant cells, in which structure of glycans of GPI is altered, the amount of GPI-AP in the plasma membrane is increased, indicating that glycan structures of GPI influences the lipid remodeling of GPI.