Protein assembly at DNA replication fork and role of replication DNA helicase

由MCM2-7解旋酶进行的DNA复制分叉是由MCM相互作用蛋白调节的。我阐明了MCM2-7与MCM相互作用蛋白的相互作用。CDC45是CMG复合体的一个组成部分，与MCM2-7的所有分子都有广泛的相互作用。同样，可能参与抑制MCM2-7复合体的MCM-BP与所有MCM2-7相互作用。这一特征对于通过MCM2-7复合体的结构变化来调节MCM的功能可能具有重要意义。其他MCM相互作用物与MCM6的ATP结合域结合，这种结合可能在MCM功能的调节中发挥作用，因为与MCM6的ATP结合是MCM功能的关键。

DNA replication fork progressed by MCM2-7 helicase is regulated by action of MCM interacting proteins. I clarified interaction of MCM2-7 with the MCM interacting proteins. CDC45 that is a component of CMG complex extensively interacted with all of MCM2-7. Similarly MCM-BP that is probably involved in inhibition of MCM2-7 complex interacted all of MCM2-7. This feature may be important for regulation of MCM function through structural change of MCM2-7 complex. Other MCM interactants bound to ATP binding domain of MCM6 and this binding may play a role in regulation of MCM function, since ATP binding toMCM6 is critical for MCM function.