Germ plasm of Xenopus is the determinant of germinal lineage

在果蝇和果蝇中，种质是决定种质谱系的决定因素。elgans。在这项工作中，我们已经证明，这也是真实的情况下，脊椎动物。非洲爪蟾种质位于卵细胞质皮层，含有母体mRNA、蛋白质和大量线粒体。我们已经做了一个转基因青蛙的线粒体是EGFP标记。由于生殖系线粒体的独特性，我们可以在一个生命周期的大部分时间内追踪GP和PGCs。当我们将GP移植到动物半球（注定成为表皮）时，我们获得了异位PGCs。异位PGCs不能从表面位置迁移，但如果它们转移到内胚层中，它们可以向性腺迁移。而且，它们可以成为功能性配子。

Germ plasm is the determinant of germinal lineage in case of Drosophila and C. elgans. In this work, we have evidenced that this is true also in case of vertebrate. Germ plasm of Xenopus is situated in the cortex of egg vegetal cytoplasm, and contains maternal mRNA, protein and a large amount of mitochondria. We have made a transgenic frog whose mitochondria is EGFP-labelled. On the behalf of unique characteristcs of germline mitochondria, we can trace GP and PGCs in most of a life cycle. When we explant GP into the animal hemisphere (fated to become epidermis), we obtain ectopic PGCs. The ectopic PGCs cannot migrate from the surface position, but they can migrate towards gonads if they are transferred into the endoderm. And, they can become functional gametes.