Ultrastructural studies on the intracellular trafficking of membranes and HCl-secreting proteins in gastric parietal cells by high-pressure freezing.

我们利用大鼠离体胃黏膜体外实验模型和高压冷冻后冷冻替代冷冻技术，研究了胃壁细胞膜和HCl筛选蛋白在细胞内的转运。通过免疫组织化学的应用，我们对壁细胞静息状态和分泌酸活性状态之间的膜融合蛋白和离子转运蛋白KCNQ1的细胞内转运有了新的认识。此外，我们在电子显微镜水平上揭示了TRIM50基因敲除小鼠胃壁细胞功能形态的独特变化。

We investigated the intracellular trafficking of membranes and HCl-screting proteins in rat gastric parietal cells by taking advantages of the ex vivo experimental model, named rat isolated gastric mucosa, and the high-pressure freezing followed by freeze substitution cryo-techniques.As results, we obtained a new insight concerning the intracellular trafficking of membrane fusion proteins, such as syntaxin3 and Hip1r, and ion-transporter channel proteins, such as KCNQ1, between the resting state and the active acid-secreting state of the parietal cell by the application of immuno-histochemistry. In addition, we revealed a unique change in functional morphology in the gastric parietal cells of TRIM50 knock-out mice at the electron microscopical level.