The elucidation of the mechanism of growth suppression of melanoma by kinase inhibitor

WNT信令通路在肿瘤发生中起着至关重要的作用。有报道表明，该信号通路在黑色素瘤中被激活。在这项研究中，我们评估了非甾体抗炎药塞来昔布是否抑制Wnt信号通路。如果塞来昔布抑制Wnt信号，该药物可作为分子靶向药物。用免疫组织化学方法检测黑色素瘤组织中β-连环蛋白的表达。一些黑色素瘤样本表达β-连环蛋白。然后，在黑色素瘤细胞的培养液中加入塞来昔布。本实验揭示了塞来昔布抑制黑色素瘤细胞的生长。研究了黑色素瘤细胞中β-连环素的含量与黑色素瘤细胞生长抑制程度的关系，提示Wnt信号的激活与黑色素瘤细胞的生长抑制效应有关。

Wnt sinaling pathway has a crucial role in tumorgenesis. Some report have demonstrated that this signaling pathway was activated in melanoma. In this study, we evaluated whether celecoxib, a NSAIDs inhibited Wnt signaling pathway or not. If celecoxib suppressed Wnt signaling, this drug can be used as a molecular targeting drug. The expression of beta-catenin in melanoma samples was assessed by immunohistochemistry. Some melanoma samples expressed beta-catenin. Next, celecoxib was administrated in cell culture medium of melanoma cells. This experiment revealed that celecoxib inhibited the growth of melanoma cells. The relationship between the amount of beta-catenin in melanoma cells and the extent of growth suppression of melanoma cells was investigated, suggesting correlation of Wnt signal activation and the effect of growth inhibition of melanoma cells.