The elucidation of the mechanism of growth suppression of melanoma by kinase inhibitor

Wnt信号转导通路在肿瘤发生中起着重要作用。有报道表明，该信号通路在黑色素瘤中被激活。在本研究中，我们评估塞来昔布，非甾体抗炎药抑制Wnt信号通路或没有。如果塞来昔布抑制Wnt信号传导，这种药物可以用作分子靶向药物。通过免疫组织化学评估黑素瘤样品中β-连环蛋白的表达。一些黑色素瘤样本表达β-连环蛋白。接下来，在黑色素瘤细胞的细胞培养基中施用塞来昔布。该实验表明，塞来昔布抑制黑色素瘤细胞的生长。研究了黑素瘤细胞中β-连环蛋白的量与黑素瘤细胞的生长抑制程度之间的关系，表明Wnt信号激活与黑素瘤细胞的生长抑制效果相关。

Wnt sinaling pathway has a crucial role in tumorgenesis. Some report have demonstrated that this signaling pathway was activated in melanoma. In this study, we evaluated whether celecoxib, a NSAIDs inhibited Wnt signaling pathway or not. If celecoxib suppressed Wnt signaling, this drug can be used as a molecular targeting drug. The expression of beta-catenin in melanoma samples was assessed by immunohistochemistry. Some melanoma samples expressed beta-catenin. Next, celecoxib was administrated in cell culture medium of melanoma cells. This experiment revealed that celecoxib inhibited the growth of melanoma cells. The relationship between the amount of beta-catenin in melanoma cells and the extent of growth suppression of melanoma cells was investigated, suggesting correlation of Wnt signal activation and the effect of growth inhibition of melanoma cells.