The elucidation of the mechanism of growth suppression of melanoma by kinase inhibitor

激酶抑制剂抑制黑色素瘤生长的机制的阐明

• 批准号: 23591614
• 负责人: HARADA Kazutoshi
• 金额: $ 3.33万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591614/
• 关键词: キナーゼ阻害薬; シグナル伝達系; 悪性黒色腫; 有棘細胞癌; 分子標的薬

Wnt sinaling pathway has a crucial role in tumorgenesis. Some report have demonstrated that this signaling pathway was activated in melanoma. In this study, we evaluated whether celecoxib, a NSAIDs inhibited Wnt signaling pathway or not. If celecoxib suppressed Wnt signaling, this drug can be used as a molecular targeting drug. The expression of beta-catenin in melanoma samples was assessed by immunohistochemistry. Some melanoma samples expressed beta-catenin. Next, celecoxib was administrated in cell culture medium of melanoma cells. This experiment revealed that celecoxib inhibited the growth of melanoma cells. The relationship between the amount of beta-catenin in melanoma cells and the extent of growth suppression of melanoma cells was investigated, suggesting correlation of Wnt signal activation and the effect of growth inhibition of melanoma cells.

Wnt信号转导通路在肿瘤发生中起着重要作用。有报道表明，该信号通路在黑色素瘤中被激活。在本研究中，我们评估塞来昔布，非甾体抗炎药抑制Wnt信号通路或没有。如果塞来昔布抑制Wnt信号传导，这种药物可以用作分子靶向药物。通过免疫组织化学评估黑素瘤样品中β-连环蛋白的表达。一些黑色素瘤样本表达β-连环蛋白。接下来，在黑色素瘤细胞的细胞培养基中施用塞来昔布。该实验表明，塞来昔布抑制黑色素瘤细胞的生长。研究了黑素瘤细胞中β-连环蛋白的量与黑素瘤细胞的生长抑制程度之间的关系，表明Wnt信号激活与黑素瘤细胞的生长抑制效果相关。

项目成果

Onychogryphosis by an elastic wire.

通过弹性线进行甲弯曲。

• DOI: 10.1111/1346-8138.12295
• 发表时间: 2013
• 期刊: J Dermatol.
• 作者: Harada K;Yamaguchi M;Watanabe S;Shimada S.
• 通讯作者: Shimada S.

Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level.

• DOI: 10.1038/bjc.2012.12
• 发表时间: 2012-02-28
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Sakaizawa, K.;Goto, Y.;Kiniwa, Y.;Uchiyama, A.;Harada, K.;Shimada, S.;Saida, T.;Ferrone, S.;Takata, M.;Uhara, H.;Okuyama, R.
• 通讯作者: Okuyama, R.

Two cases of autosomal recessive woolly hair with LIPH gene mutations

常染色体隐性羊毛状毛发LIPH基因突变2例

• DOI: 10.1111/j.1365-4632.2012.05775.x
• 发表时间: 2013
• 期刊: Int J Dermatol
• 影响因子: 3.6
• 作者: Harada K;Inozume T;Kawamura T;Shibagaki N;Kinoshita T;Deguchi N;Shimada S
• 通讯作者: Shimada S

A patient with pseudochromhidrosis presenting with pink nails

假色汗症患者的指甲呈粉红色

• DOI: 10.1016/j.jaad.2011.08.026
• 发表时间: 2012
• 期刊: J. Am. Acad. Dermatol
• 作者: K. Harada;T. Morohoshi;T. Ikeda;and S. Shimada
• 通讯作者: and S. Shimada

Marked response to imatinib mesylate in metastatic acral lentiginous melanoma on the thumb

甲磺酸伊马替尼对拇指转移性肢端雀斑样黑色素瘤有显着疗效

• DOI: 10.1111/j.1365-2230.2010.03885.x
• 发表时间: 2011
• 期刊: Clin Exp Dermatol
• 影响因子: 4.1
• 作者: Yamaguchi M;Harada K;Ando N;Kawamura T;Shibagaki N;Shimada S
• 通讯作者: Shimada S