Regenerative pancreatic islet transplantation for the young onset Type I diabetes mellitus. It is xenotransplantation of the pancreatic islet cell sheet.

再生胰岛移植治疗年轻发病的 I 型糖尿病。

• 批准号: 23591871
• 负责人: ISHII Show
• 金额: $ 3.16万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591871/
• 关键词: 細胞シート; I型糖尿病; 膵島移植; 膵島再生; ティッシュエンジニアリング; 異種移植; 組織工学

The present study was conducted to develop a dispersed islet cell-based tissue engineering approach for the treatment of diabetes mellitus. Dispersed islet cells obtained from rats were then plated on the laminin-5-PIPAAm dishes. At day 2 of culture, cultured islet cells were harvested as a uniformly connected tissue sheet by lowering the culture temperature from 37 degrees C to 20 degrees C for 30 min. Two harvested islet cell sheets were transplanted for engineering neo-islet tissues in the subcutaneous site of streptozotocin-induced diabetic mice. The diabetic mice that received islet sheet transplantation did not revert from hyperglycemia. Histological examination revealed that the transplanted islet cell sheets formed clusters of islet tissues and inflammatory cell infiltration. The present study may describe a new proof-of-concept therapeutic approach to engineer functional neo-islet tissues for xenotransplantation.

本研究旨在开发一种基于分散胰岛细胞的组织工程方法来治疗糖尿病。然后将从大鼠获得的分散的胰岛细胞铺在层粘连蛋白-5-PIPAAm培养皿上。 在培养第2天，通过将培养温度从37℃降低至20℃30分钟，将培养的胰岛细胞收获为均匀连接的组织片。 将两个收获的胰岛细胞片移植到链脲佐菌素诱导的糖尿病小鼠的皮下部位，用于改造新胰岛组织。接受胰岛片移植的糖尿病小鼠并未从高血糖中恢复。 组织学检查显示，移植的胰岛细胞片形成胰岛组织簇和炎症细胞浸润。本研究可能描述了一种新的概念验证治疗方法，用于设计用于异种移植的功能性新胰岛组织。

项目成果

Long-term outcome of clinical islet transplantation using donors after cardiac death : a multicenter experience in Japan

心脏死亡后使用供体进行临床胰岛移植的长期结果：日本的多中心经验

• 发表时间: 2013
• 作者: Anazawa T;Kenjo A;Kimura T;Ise K;Haga J;Sato N;Tsuchiya T;Sato T;Gotoh M.
• 通讯作者: Gotoh M.

Mitomycin-C treatment protects islets from the progression of central damage during culture.

丝裂霉素-C 治疗可保护胰岛在培养过程中免受中央损伤的进展。

• 发表时间: 2011
• 作者: Haga J;Saito T;Anazawa T;Tsuchiya T;Kenjo A;Sato S;Sato N;Saito T;Ise K;Yamamoto M;Waguri S;Gotoh M
• 通讯作者: Gotoh M

Topographical arrangement of α- and β-cells within neo-islet tissues engineered by islet cell sheet transplantation in mice.

通过小鼠胰岛细胞片移植工程改造的新胰岛组织内 α 和 β 细胞的拓扑排列。

• DOI: 10.1016/j.transproceed.2013.01.003
• 发表时间: 2013
• 期刊: Transplantation Proceedings
• 影响因子: 0.9
• 作者: Shimizu H;Ohashi K;Saito T;Utoh R;Ise K;Yamato M;Okano T;Gotoh M.
• 通讯作者: Gotoh M.

Long-term follow-up of clinical islet transplantation using donors after cardiac death: Report of the Japan Islet Transplantation Registry

心脏死亡后使用供体进行临床胰岛移植的长期随访：日本胰岛移植登记处的报告

• 发表时间: 2013
• 作者: Junichiro Haga;Takayuki Anazawa;穴澤 貴行;穴澤 貴行;穴澤 貴行;Takayuki Anazawa
• 通讯作者: Takayuki Anazawa

膵島移植の新たな展開

胰岛移植的新进展

• 发表时间: 2011
• 作者: 清水裕史;大橋一夫;斎藤敬弘;鵜頭理恵;伊勢一哉;大和雅之;岡野光夫;後藤満一
• 通讯作者: 後藤満一