Global analysis of genomic alterations associated with AKT and ERK pathway in esophageal squamous cell carcinoma

食管鳞状细胞癌中与 AKT 和 ERK 通路相关的基因组改变的整体分析

• 批准号: 23591941
• 负责人: MIYANARI Nobutomo
• 金额: $ 3.41万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591941/
• 关键词: genomic alteration; 食道扁平上皮癌; KRAS; BRAF; PIK3CA; 遺伝子変異; 同時性肝転移; 食道癌; 予後; pyrosequence

Using resected ESCC specimens,we investigate mutations of KRAS,BRAF and PIK3CA by Pyrosequencing technology.As a result,the KRAS mutation was found to be extremely rare(1 of 203;0.5%),and the BRAF mutation was absent(0 of 203;0%),suggesting that KRAS and BRAF mutations play a limited role in the development of ESCC.However,PIK3CA mutations mutations in ESCC are associated with longer overall survival and cancer-specific survival,suggesting its role as a prognostic biomarker.We evaluated colorectal cancer with synchronous liver metastases. We evaluated KRAS,BRAF,and PIK3CA mutation status and microsatellite instability(MSI) status in 42 liver metastases, 26 matched primary tumors, and 6 matched lymph node(LN) metastases by Pyrosequencing technology.We also quantified LINE-1 methylation levels.The concordance rate was 94% for KRAS,100% for BRAF,88% for PIK3CA,and 97% for MSI.Primary tumors showed LINE-1 methylation levels similar to those of matched liver and LN metastases.

本研究利用焦磷酸测序技术对食管鳞癌组织中KRAS、BRAF和PIK 3CA基因突变进行了研究，结果发现KRAS基因突变极为罕见（1/203;0.5%），BRAF突变缺失（0/203）提示KRAS和BRAF突变在ESCC的发生中作用有限。ESCC中PIK 3CA突变与较长的总生存期和癌症特异性生存期相关，表明其作为预后生物标志物的作用。我们通过焦磷酸测序技术检测了42例肝转移瘤、26例匹配原发肿瘤和6例匹配淋巴结转移瘤的KRAS、BRAF和PIK 3CA突变状态和微卫星不稳定性（MSI）状态，并定量了LINE-1甲基化水平。KRAS、BRAF和PIK 3CA的一致率分别为94%、100%、88%，原发肿瘤显示LINE-1甲基化水平与匹配的肝和LN转移瘤相似。

项目成果

MIcroRNA-200B regulates epithelial to mesenchymal transition in gastric carcinoma

MIcroRNA-200B 调节胃癌上皮间质转化

• 发表时间: 2011
• 作者: Junji Kurashige ;Masayuki Watanabe;Yukiharu Hiyoshi ;Hidenobu Kamohara ;Koichi Kinoshita;Seiya Saito;Masaaki Iwatsuki;Yoshifumi Baba ;Naoko Hayashi ;Hideo Baba;Kurashige J;Kinoshita K;Nakahara O;Kurashige
• 通讯作者: Kurashige

MicroRNA-29c expression as a new prognostic marker for esophageal squamous cell carcinoma

MicroRNA-29c表达作为食管鳞状细胞癌新的预后标志物

• 发表时间: 2011
• 作者: Junji Kurashige ;Masayuki Watanabe;Yukiharu Hiyoshi ;Hidenobu Kamohara ;Koichi Kinoshita;Seiya Saito;Masaaki Iwatsuki;Yoshifumi Baba ;Naoko Hayashi ;Hideo Baba;Kurashige J;Kinoshita K
• 通讯作者: Kinoshita K

Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma

• DOI: 10.1002/jso.23064
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF SURGICAL ONCOLOGY
• 影响因子: 2.5
• 作者: Kurashige, Junji;Kamohara, Hidenobu;Baba, Hideo
• 通讯作者: Baba, Hideo

Carcinogenesis of Intraductal Papillary Mucinous Neoplasm of the Pancreas: Loss of MicroRNA-101 Promotes Overexpression of Histone Methyltransferase EZH2

• DOI: 10.1245/s10434-011-2068-6
• 发表时间: 2012-07-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Nakahara, Osamu;Takamori, Hiroshi;Baba, Hideo
• 通讯作者: Baba, Hideo