Global analysis of genomic alterations associated with AKT and ERK pathway in esophageal squamous cell carcinoma

食管鳞状细胞癌中与 AKT 和 ERK 通路相关的基因组改变的整体分析

• 批准号: 23591941
• 负责人: MIYANARI Nobutomo
• 金额: $ 3.41万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591941/
• 关键词: genomic alteration; 食道扁平上皮癌; KRAS; BRAF; PIK3CA; 遺伝子変異; 同時性肝転移; 食道癌; 予後; pyrosequence

Using resected ESCC specimens,we investigate mutations of KRAS,BRAF and PIK3CA by Pyrosequencing technology.As a result,the KRAS mutation was found to be extremely rare(1 of 203;0.5%),and the BRAF mutation was absent(0 of 203;0%),suggesting that KRAS and BRAF mutations play a limited role in the development of ESCC.However,PIK3CA mutations mutations in ESCC are associated with longer overall survival and cancer-specific survival,suggesting its role as a prognostic biomarker.We evaluated colorectal cancer with synchronous liver metastases. We evaluated KRAS,BRAF,and PIK3CA mutation status and microsatellite instability(MSI) status in 42 liver metastases, 26 matched primary tumors, and 6 matched lymph node(LN) metastases by Pyrosequencing technology.We also quantified LINE-1 methylation levels.The concordance rate was 94% for KRAS,100% for BRAF,88% for PIK3CA,and 97% for MSI.Primary tumors showed LINE-1 methylation levels similar to those of matched liver and LN metastases.

使用切除的ESCC样品，我们通过焦磷酸测序技术研究KRAS，BRAF和PIK3CA的突变。结果，发现KRAS突变极为罕见（203; 0.5％中的1个； 0.5％），并且不存在BRAF突变（203; 0％的0），表明KRAS和BRAF突变在较长的角色中起着ESCC的较长的作用。总体生存和癌症特异性生存，表明其作为预后生物标志物的作用。我们用同步肝转移评估了结直肠癌。我们评估了KRAS，BRAF和PIK3CA突变状态和微卫星不稳定性（MSI）状态（MSI）在42个肝转移，26个匹配的原发性肿瘤和6个匹配的淋巴结（LN）转移酶中，通过pyrosequencing Technology进行。我们还量化了甲基化水平94％的甲基化速率，为94％的甲基化率，88％的甲基化水平。 MSI。主要肿瘤显示与匹配的肝脏和LN转移相似的甲基化水平。

项目成果

MIcroRNA-200B regulates epithelial to mesenchymal transition in gastric carcinoma

MIcroRNA-200B 调节胃癌上皮间质转化

• 发表时间: 2011
• 作者: Junji Kurashige ;Masayuki Watanabe;Yukiharu Hiyoshi ;Hidenobu Kamohara ;Koichi Kinoshita;Seiya Saito;Masaaki Iwatsuki;Yoshifumi Baba ;Naoko Hayashi ;Hideo Baba;Kurashige J;Kinoshita K;Nakahara O;Kurashige
• 通讯作者: Kurashige

MicroRNA-29c expression as a new prognostic marker for esophageal squamous cell carcinoma

MicroRNA-29c表达作为食管鳞状细胞癌新的预后标志物

• 发表时间: 2011
• 作者: Junji Kurashige ;Masayuki Watanabe;Yukiharu Hiyoshi ;Hidenobu Kamohara ;Koichi Kinoshita;Seiya Saito;Masaaki Iwatsuki;Yoshifumi Baba ;Naoko Hayashi ;Hideo Baba;Kurashige J;Kinoshita K
• 通讯作者: Kinoshita K

Carcinogenesis of Intraductal Papillary Mucinous Neoplasm of the Pancreas: Loss of MicroRNA-101 Promotes Overexpression of Histone Methyltransferase EZH2

• DOI: 10.1245/s10434-011-2068-6
• 发表时间: 2012-07-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Nakahara, Osamu;Takamori, Hiroshi;Baba, Hideo
• 通讯作者: Baba, Hideo

Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma

• DOI: 10.1002/jso.23064
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF SURGICAL ONCOLOGY
• 影响因子: 2.5
• 作者: Kurashige, Junji;Kamohara, Hidenobu;Baba, Hideo
• 通讯作者: Baba, Hideo