The study on functions of opioid receptors expressed in T lymphocytes

T淋巴细胞表达的阿片受体功能研究

• 批准号: 23592252
• 负责人: SHODA Takehiro
• 金额: $ 3.24万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592252/
• 关键词: 麻薬; モルヒネ; Tリンパ球; オピオイド; 免疫抑制; 血管平滑筋; オピオイド受容体; T細胞; 血管弛緩

Narcotics have been reported to develop immunosuppression as one of their side effects, the mechanism of which might be involved in opioid receptors expressed on immune cells, such as T lymphocytes.We have cloned before, from human T cell cDNA library, the cDNA encoding a splice variant of kappa opioid receptor, truncated at its amino terminus (termed as KOR4). The stable transformants of HEK293 cells heterologously transfected with KOR4 cDNA are now being constructed and characterization of KOR4 will be investigated. Moreover, we have demonstrated that, in human T lymphocytes and Jurkat cells, signaling pathways by T cell receptor stimulation would be alterd by morphine pretreatment. These results suggest that narcotics could change T cell functions and modulate immune systems.

据报道，麻醉药物会产生免疫抑制作用，其机制可能与T淋巴细胞等免疫细胞上表达的阿片受体有关。我们之前从人T细胞cDNA文库中克隆了编码κ阿片受体剪接变体的cDNA，其氨基末端被截短（称为KOR4）。目前正在构建用 KOR4 cDNA 异源转染的 HEK293 细胞的稳定转化体，并将研究 KOR4 的表征。此外，我们已经证明，在人 T 淋巴细胞和 Jurkat 细胞中，吗啡预处理会改变 T 细胞受体刺激的信号通路。这些结果表明麻醉剂可以改变 T 细胞功能并调节免疫系统。

项目成果

Morphine induces DNA damage and P53 activation in CD3+ T cells.

• DOI: 10.1016/j.bbagen.2009.04.011
• 发表时间: 2009-08
• 期刊: Biochimica et biophysica acta
• 作者: H. Tsujikawa;T. Shoda;T. Mizota;K. Fukuda