SNP typing of the cariogenic protein gene

致龋蛋白基因的SNP分型

• 批准号: 23592790
• 负责人: MATSUDA Yasuhiro
• 金额: $ 3.16万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592790/
• 关键词: 遺伝子多型; 遺伝子分析; う蝕感受性; 唾液緩衝能; 齲蝕; PCR高解像度融解曲線分析

To evaluate the discrimination method of HLA Alleles, we developed the detection method by PCR-Sequence Specific Primer (SSP) and the melt peak analysis (Tm). The frequencies of target Allele calculated from database were less than 10%, therefore our method could discriminate more than 90% samples.We analyzed the Buffer capacity of saliva and Carbonic Anhydrase VI SNP of Children attended permanent caries preventing program. There were a positive association between caries present and buffer capacity. We could distinguish the rs2274327 (C/T) polymorphism by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method.

为了评估HLA等位基因的鉴别方法，我们建立了PCR-Sequence Specific Primer （SSP）和熔融峰分析（Tm）检测方法。从数据库中计算的目标等位基因的频率小于10%，因此我们的方法可以区分90%以上的样本。我们分析了参加永久性预防龋齿项目的儿童唾液的缓冲容量和碳酸酐酶VI SNP。存在的龋齿与缓冲能力之间存在正相关。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对rs2274327 （C/T）基因多态性进行了鉴别。

项目成果

Comparison of Fluoride Regimens on Fluorine Uptake in Carious Enamel

氟化物方案对龋齿釉质氟吸收的比较

• 发表时间: 2013
• 作者: Naoki HASHIMOTO;Yoshiki FUNATO;Saiko OKI;Yasuhiro. MATSUDA;Katsushi OKUYAMA;Hiroko YAMAMOTO;Hisanori KOMATSU;and Hidehiko SANO
• 通讯作者: and Hidehiko SANO

Effective Screening for the HLA-B*35:05 and HLA-B*57:01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay

使用等位基因特异性聚合酶链反应熔解测定有效筛选 HLA-B*35:05 和 HLA-B*57:01 等位基因

• 发表时间: 2014
• 期刊: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013)
• 作者: Yasuhiro Matsuda;Hidehiko Sano;Yuichi Iwaki
• 通讯作者: Yuichi Iwaki

う蝕と遺伝子解析

龋齿与遗传分析

• 发表时间: 2013
• 期刊: 北海道歯誌
• 作者: Han L;Kodama S;Okiji T;Kamada A.;松田康裕
• 通讯作者: 松田康裕

Effective Screening for the HLA-B*35: 05 and HLA-B*57: 01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay

使用等位基因特异性聚合酶链式反应熔解测定有效筛选 HLA-B*35: 05 和 HLA-B*57: 01 等位基因

• 发表时间: 2013
• 期刊: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013)
• 作者: Yasuhiro Matsuda;Hidehiko Sano;Yuichi Iwaki
• 通讯作者: Yuichi Iwaki

Demineralize Prevention of Dentin With Fluoride Varnish via Automatic pH-cycling

通过自动 pH 循环使用氟化物清漆预防牙本质脱矿质

• 发表时间: 2013
• 作者: Saiko OKI;Yasuhiro MATSUDA;Naoki HASHIMOTO;Katsushi OKUYAMA;Hiroko YAMAMOTO;Yoshiki FUNATO;Hisanori KOMATSU;and Hidehiko SANO
• 通讯作者: and Hidehiko SANO