Development of new therapy of Sjogren's syndrome ; by using with cytokine and regenerative medicine

干燥综合征（SS）是一种系统性自身免疫性疾病，涉及唾液和泪液分泌减少，然而，唾液分泌减少的机制尚不清楚。在SS患者的唾液腺中，存在于根尖质膜的主要水通道AQP - 5的表达减少，AQP - 5从根尖到基膜分布异常。AQP5在SS唾液腺中的作用机制尚不完全清楚。研究人员发现，在DNA去甲基化剂5-aza-2 ' -脱氧胞苷（5-Aza-CdR）的作用下，缺乏AQP5表达的正常人唾液腺导管细胞（NS-SV-DC）获得了AQP5基因的表达。表达的AQP5蛋白具有功能活性。我们研究了体外和体内诱导AQP5的机制，讨论了一种DNA去甲基化剂可能是一种有用的药物，可以恢复老年人的低唾液，从而导致口干症的解决。

Sjogren's syndrome (SS) is a systemic autoimmune disease that involves a reduction in salivary and lacrimal secretions, however, the mechanisms underlying the decrease in salivary secretion remains unclear. In salivary glands of SS patients, the expression of aquaporin (AQP) 5, a major water channel present in the apical plasma membrane, was decreased and aberrant distribution of AQP5 from apical to basal membrane was reported. The mechanisms of AQP5 dysfunction in SS salivary glands have not yet fully understood. We demonstrate that an immortalized normal human salivary gland ductal cell (NS-SV-DC) line, lacking the expression of AQP5, acquires AQP5 gene expression in response to treatment with 5-aza-2’-deoxycytidine (5-Aza-CdR), a DNA demethylating agent. The expressed AQP5 protein was functionally active. We examined the mechanisms of induction of AQP5 in vitro and in vivo, to discuss suggests that a DNA demethylating agent may be a useful drug for restoring hyposalivation in elderly individuals, thereby leading to the resolution of xerostomia.