A new method to evaluate the function of mitotic kinases by monitoring chromosome dynamics

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Mitotic kinases play crucial roles in separating chromosomes correctly and safely as cells divide. Dysfunction of mitotic kinases therefore causes defective chromosome movements and segregation. In this study, we aimed to develop an assay to indicate cryptic dysfunction of mitotic kinases by monitoring chromosome behavior during mitosis, which is otherwise difficult to detect in conventional means. We found that increasing dose of specific inhibitors of mitotic kinases, caused abnormal chromosomal movements progressively. This what we called the stepwise-challenging assay was then used to address the function of mitotic kinases in several cancer cells that are known to have chromosomal instability