Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis研究代表者

将间充质干细胞直接移植到患有自发性骨关节炎的Hartley品系豚鼠的膝关节中主要研究员

基本信息

批准号：
23791632
负责人：
MIYAZAKI Tsuyoshi
金额：
$ 2.75万
依托单位：
University of Fukui
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791632/
关键词：
骨軟骨代謝学幹細胞軟骨再生変形性関節症

项目摘要

Mesenchymal stem cells (MSCs) can differentiate into various connective tissue cells. Several techniques have been used for the clinical application of MSCs in articular cartilage repair; however, there are many issues associated with the selection of the scaffold material, including its ability to support cell viability and differentiation and its retention and degradation in situ. The application of MSCs via a scaffold also requires a technically demanding surgical procedure. The aim of this study was to test the outcome of intra-articular transplantation of mesenchymal stem cells suspended in hyaluronic acid (HA) in the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis (OA). At 5 weeks post transplantation, partial cartilage repair was noted in the HA-MSC group but not in the other groups. Examination of CFDA-SE-labeled cells demonstrated migration, differentiation, and proliferation of MSC in the HA-MSC group. There was strong immunostaining for type II collagen around both residual chondrocytes and transplanted MSCs in the OA cartilage. Conclusion: This scaffold-free and technically undemanding technique appears to result in the regeneration of articular cartilage in the spontaneous OA animal model. Although further examination of the long-term effects of transplantation is necessary, the findings suggest that intra-articular injection of HA-MSC mixture is potentially beneficial for OA.

间充质干细胞（MSC）可以分化为各种结缔组织细胞。 MSC在关节软骨修复中的临床应用已使用。但是，与脚手架材料的选择有关，包括支持细胞生存能力和分化的能力及其原位保留和降解的能力。 MSC通过脚手架的应用还需要技术要求的手术程序。这项研究的目的是测试悬浮在透明质酸（HA）中的间充质干细胞的关节内移植的结局，该疗程中的透明质酸（HA）与自发性骨关节炎（OA）的Hartley菌株豚鼠的膝关节关节中。移植后第5周，HA-MSC组有部分软骨修复，但在其他组中则没有。对CFDA-SE标记的细胞的检查表明，HA-MSC组中MSC的分化和扩散。在OA软骨中，在残留的软骨细胞和移植的MSC周围，II型胶原蛋白具有强烈的免疫染色。结论：这种无脚手架和技术上不符合的技术似乎导致自发OA动物模型中关节软骨的再生。尽管有必要进一步检查移植的长期影响，但发现表明关节内注射HA-MSC混合物可能对OA有益。