Development of the effective bone augmentation using intracellular transfection of anti-apoptosis protein HSP27

利用细胞内转染抗凋亡蛋白HSP27开发有效的骨增量

• 批准号: 23792215
• 负责人: KAWASAKI Maiko
• 金额: $ 2.66万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792215/
• 关键词: HSP27; 骨芽細胞; アポトーシス; トランスフェクション法; 骨増成; 細胞移植

In the present study, to explore a new way of cell transplantation for bone augmentation, we evaluate the application of HSP27, an anti-apoptotic protein to the transplanted cell. The over expression of HSP27 did not affect the cell proliferation and mineralization ability of osteoblast, however, apoptosis was diminished by the over expression of HSP27. Furthermore, transplanted cell apoptosis was also inhibited and proliferation marker expressing cells were increased at the initial stage of cell transplantation.

在本研究中，我们评估了一种抗凋亡蛋白HSP27在移植细胞中的应用，以探索一种新的细胞移植方法。HSP27的过度表达不影响成骨细胞的增殖和矿化能力，但HSP27的过度表达可减少细胞的凋亡。此外，在细胞移植初期，移植细胞的凋亡也受到抑制，表达增殖标记物的细胞数量增加。

项目成果

The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling.

• DOI: 10.1186/1741-7007-11-27
• 发表时间: 2013-03-28
• 期刊: BMC biology
• 影响因子: 5.4
• 作者: Khonsari RH;Seppala M;Pradel A;Dutel H;Clément G;Lebedev O;Ghafoor S;Rothova M;Tucker A;Maisey JG;Fan CM;Kawasaki M;Ohazama A;Tafforeau P;Franco B;Helms J;Haycraft CJ;David A;Janvier P;Cobourne MT;Sharpe PT
• 通讯作者: Sharpe PT

Cytoplasmic plaque formation in hemidesmosome development is dependent on SoxF transcription factor function.

• DOI: 10.1371/journal.pone.0043857
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Oommen S;Francois M;Kawasaki M;Murrell M;Kawasaki K;Porntaveetus T;Ghafoor S;Young NJ;Okamatsu Y;McGrath J;Koopman P;Sharpe PT;Ohazama A
• 通讯作者: Ohazama A