Relationship between metastasis of cancer and abnormality of tumor endothelial cell

癌症转移与肿瘤内皮细胞异常的关系

• 批准号: 23792306
• 负责人: NORITAKA Ohga
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792306/
• 关键词: 腫瘍血管新生; 転移; 多様性; 薬剤抵抗性; 幹細胞性; 腫瘍血管内皮細胞; 腫瘍血管

It is an important concept of tumor angiogenesis that tumor endothelial cells (TEC) are genetically normal and homogeneous. However, we had previously reported that TEC differ from normal endothelial cells (NEC). Whether the characteristics of TEC derived from different tumors differ remains unknown. To elucidate this, we isolated two types of TEC from highly metastatic tumor and low metastatic tumor and compared their characteristics. High metastatic tumor-derived TEC (HM-TEC) showed higher proliferative activity and invasive activity than low metastatic tumor-derived TEC (LM-TEC). Moreover, the mRNA expression levels of proangiogenic genes, such as VEGF-R1 and 2, VEGF and hypoxia (HIF-1) were higher in HM-TEC compared with those in LM-TEC. Furthermore, HM-TEC showed higher mRNA expression levels of the stemness-related genes compared with LM-TEC. These results indicate that TEC from high metastatic tumors have developed more abnormality than those from low metastatic tumors.

肿瘤内皮细胞(TEC)在遗传学上是正常的、同质的，这是肿瘤血管生成的一个重要概念。然而，我们先前报道了TEC不同于正常内皮细胞(NEC)。目前尚不清楚不同肿瘤来源的TEC是否具有不同的特性。为了阐明这一点，我们从高转移肿瘤和低转移肿瘤中分离出两种类型的TEC，并比较了它们的特性。高转移肿瘤来源的TEC(HM-TEC)比低转移肿瘤来源的TEC(LM-TEC)具有更高的增殖活性和侵袭活性。此外，HM-TEC中促血管生成基因VEGF-R1和2、血管内皮生长因子和缺氧(HIF-1)的mRNA表达水平均高于LM-TEC。此外，与LM-TEC相比，HM-TEC表现出更高的茎粗相关基因的表达水平。这些结果表明，高转移性肿瘤的TEC比低转移性肿瘤的TEC有更多的异常。

项目成果

Hypoxia -induced reactive oxygen species cause chromosomal abnormality of endothelial cells in tumor microenvironment

缺氧诱导的活性氧导致肿瘤微环境内皮细胞染色体异常

• 发表时间: 2013
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: Kondoh.M;Ohga.N.;Akiyama.K;Hida.Y;Maishi.N;Alam Mohammad Towfik;Inoue.N;Shindoh.M;Hida.K.
• 通讯作者: Hida.K.

Comparative characterization of tumor endothelial cells isolated from highly and low metastatic tumors

从高转移性和低转移性肿瘤中分离的肿瘤内皮细胞的比较特征

• 发表时间: 2011
• 作者: Ishikawa S.;Hida Y.;Akiyama K.;Kitayama K.;Kondoh M.;Maishi N.;Kawamoto T.;Ohsawa T.;Yamamoto K.;Shindoh M.;Hida K.
• 通讯作者: Hida K.

前立腺とカテキン

前列腺和儿茶素

• 发表时间: 2011
• 作者: 大賀則孝;樋田京子
• 通讯作者: 樋田京子

Heterogeneity of Tumor Endothelial Cells : Comparison between Tumor Endothelial Cells Isolated from Highly Metastatic and Low Metastatic Tumors

肿瘤内皮细胞的异质性：高转移性和低转移性肿瘤分离的肿瘤内皮细胞的比较

• 发表时间: 2012
• 期刊: Am J Pathol
• 影响因子: 6
• 作者: Ohga N.;Ishikawa S.;Maishi N.;Akiyama K.;Hida Y.;Kawamoto T.;Sadamoto Y.;Osawa T.,Yamamoto K.;Kondoh M.;Ohmura H.;Shinohara N.;Nonomura K.;Shindoh M.;Hida K
• 通讯作者: Hida K

転移能の異なる腫瘍由来の血管内皮の特性解析

具有不同转移潜能的肿瘤来源的血管内皮的表征

• 作者: 3.大賀則孝;石川修平;樋田泰浩;秋山廣輔;間石奈湖;川本泰輔;進藤正信;樋田京子
• 通讯作者: 樋田京子