Identification and functional analysis of novel bioactive peptides that regulate energy metabolism and food intake

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We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey assay). Candidate ligands in tissue extracts were screened against cell lines expressing target orphan receptors by using this system. In cells expressing a certain orphan receptor, we detected the significant increase of impedance, which was the target receptor specific. We are now proceeding to purify the active peptide from this extract.