Identification and functional analysis of novel bioactive peptides that regulate energy metabolism and food intake

我们进行了一项研究，寻找作用于下丘脑中表达的孤儿受体的新的生物活性多肽，该受体控制能量代谢和摄食行为。为了筛选这些受体的候选配体，我们开发了一种新的活性检测系统，该系统通过细胞的微观形态变化来确定活性(CellKey实验)。利用该系统对组织提取液中的候选配体与表达靶孤儿受体的细胞系进行筛选。在表达特定孤儿受体的细胞中，我们检测到显著增加的阻抗，这是目标受体特异性的。我们现在正在从这个提取物中提纯活性多肽。

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey assay). Candidate ligands in tissue extracts were screened against cell lines expressing target orphan receptors by using this system. In cells expressing a certain orphan receptor, we detected the significant increase of impedance, which was the target receptor specific. We are now proceeding to purify the active peptide from this extract.