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寄生植物ストライガのトランスクリプトーム解析

寄生植物独脚金的转录组分析

基本信息

批准号：
12F02781
负责人：
白須賢
金额：
$ 1.47万
依托单位：
The Institute of Physical and Chemical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2012
资助国家：
日本
起止时间：
2012 至 2014
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12F02781/
关键词：
Parastic Plants RNA-Sequencing Striga Pectin-methyl-esterase Inhibitor 植物病理学生理学遺伝子

项目摘要

I developed two projects to elucidate molecular interaction between host plants and parasitic plants. I focus thereby of three different parasitic plants, Striga asiatica, Striga hermonthica and Phtheirospermum japonicum.1) I characterized of a novel protein family of secreted proteins, which are conserved in at least three parasitic plants species and carry a Plant invertase/pectin methylesterase inhibitor domain. My new results show that members of this protein family specifically localized to haustoria at late stages of infection and that expression of these genes in non-parasitic plants caused to severe developmental phenotypes. These phenotypes maybe associated with altered cell wall composition or nutrient acquisition.2) I studied host hormone responses during plant parasitism and discovered an involvement of host auxin signalling components in infection and haustoria development. I established a method to evaluate Arabidopsis infection by S. asiatica, S. hermonthica and P. japonicum and screened a collection of known Arabidopsis auxin signalling mutants for resistance against these parasitic plants. I discovered that mutants along the Solitary Root (SLR) pathway exhibited elevated levels of resistance. This work showed that parasitic plants couple infection processes to host components such as auxin signalling.

我开发了两个项目来阐明寄主植物和寄生植物之间的分子相互作用。因此，我聚焦于三种不同的寄生植物，Striga asiatica，Striga hermonthica和Phtheirospermum japonicum。1）我的特征在于分泌蛋白的新蛋白质家族，其在至少三种寄生植物物种中是保守的，并且携带植物转化酶/果胶甲酯酶抑制剂结构域。我的新结果表明，该蛋白质家族的成员在感染后期特异性地定位于吸器，并且这些基因在非寄生植物中的表达导致严重的发育表型。这些表型可能与改变细胞壁组成或营养获取有关。2）我研究了植物寄生过程中宿主激素的反应，发现宿主生长素信号成分参与了侵染和吸器发育。本研究建立了一种检测S. asiatica、S. hermonthica和P. japonicum中的一种，并筛选了已知的拟南芥生长素信号传导突变体的集合以抵抗这些寄生植物。我发现沿着孤根（SLR）途径的突变体沿着表现出更高的抗性水平。这项工作表明，寄生植物耦合感染过程的主机组件，如生长素信号。