寄生植物ストライガのトランスクリプトーム解析

寄生植物独脚金的转录组分析

• 批准号: 12F02781
• 负责人: 白須 賢
• 金额: $ 1.47万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 2014
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12F02781/
• 关键词: Parastic Plants; RNA-Sequencing; Striga; Pectin-methyl-esterase Inhibitor; 植物; 病理学; 生理学; 遺伝子

I developed two projects to elucidate molecular interaction between host plants and parasitic plants. I focus thereby of three different parasitic plants, Striga asiatica, Striga hermonthica and Phtheirospermum japonicum.1) I characterized of a novel protein family of secreted proteins, which are conserved in at least three parasitic plants species and carry a Plant invertase/pectin methylesterase inhibitor domain. My new results show that members of this protein family specifically localized to haustoria at late stages of infection and that expression of these genes in non-parasitic plants caused to severe developmental phenotypes. These phenotypes maybe associated with altered cell wall composition or nutrient acquisition.2) I studied host hormone responses during plant parasitism and discovered an involvement of host auxin signalling components in infection and haustoria development. I established a method to evaluate Arabidopsis infection by S. asiatica, S. hermonthica and P. japonicum and screened a collection of known Arabidopsis auxin signalling mutants for resistance against these parasitic plants. I discovered that mutants along the Solitary Root (SLR) pathway exhibited elevated levels of resistance. This work showed that parasitic plants couple infection processes to host components such as auxin signalling.

我开发了两个项目来阐明寄主植物和寄生植物之间的分子相互作用。1)I是一个新的分泌蛋白家族，在至少三种寄生植物中保守，并携带植物转氨酶/果胶甲酯酶抑制域。我的新结果表明，这个蛋白家族的成员在感染后期专门定位于吸器，这些基因在非寄生植物中的表达导致了严重的发育表型。这些表型可能与细胞壁成分改变或营养获取有关。2)我研究了寄主激素在植物寄生过程中的反应，发现寄主生长素信号成分参与了侵染和吸器的发育。我建立了一种方法来评估拟南芥对亚洲拟南芥、赫氏拟南芥和日本拟南芥的侵染，并筛选了一组已知的拟南芥生长素信号突变体对这些寄生植物的抗性。我发现沿着孤根(SLR)途径的突变体表现出更高的抗性水平。这项工作表明，寄生植物将感染过程与生长素信号等宿主成分结合起来。