寄生植物における吸器誘導のシグナル伝達系の解明

在2021年，我鉴定了吸器诱导化合物（HIF） 2,6二甲氧基苯醌（DMBQ）处理后诱导的内源性日本血吸虫肽激素。我发现用内源性肽处理日本血吸虫诱导吸器形成而不使用hif处理，并诱导了已知吸器形成标记基因PjYUC3的表达。进一步的研究表明，在日本血吸虫基因组中还有3种推测的肽激素和6种推测的肽受体。进一步的研究发现，在DMBQ处理后，这三种肽中只有一种被上调，而在DMBQ处理后，没有受体被上调。我进一步利用表达报告基因构建体的转化日本血吸虫毛状根对肽和受体的表达进行了表征。我发现在DMBQ处理后，只有四种肽中的一种和可能的三种受体在吸器中表达。最后，过多的（PLT）转录因子在翻译后被肽稳定。通过毛状根转化，我发现DMBQ处理导致PjPLT蛋白沿着根向上移动，并且这个PjPLT定位在吸器中。

During 2021, I identified endogenous Phtheirospermum japonicum peptide hormones that are induced upon treatment with the haustorium inducing compound (HIF) 2,6 dimethoxybenzoquinone (DMBQ). I found that treatment of P. japonicum with an endogenous peptide induced haustorium formation without the treatment of an HIFs and induced expression of the known haustorium formation marker gene PjYUC3. Further investigation revealed that there are three additional putative peptide hormones in the P. japonicum genome and six putative peptide receptors. Further investigation found that only one of those three peptides is up-regulated following DMBQ treatment while none of the receptors are up-regulated following DMBQ treatment. I further characterized the expression of the peptides and receptors using transformed P. japonicum hairy roots expressing reporter gene constructs. I found that only one of the four peptides and potentially three of the receptors are expressed in the haustorium following DMBQ treatment. Lastly, PLETHORA (PLT) transcription factors are stabilized post-translationally by the peptides. Using hairy root transformation, I found that DMBQ treatment resulted in movement of a PjPLT protein up the root and that this PjPLT localized in the haustorium.