The effects of the amino acid profile in tear fluid on ocular surface homeostasis and disease condition

泪液中氨基酸谱对眼表稳态和疾病状况的影响

• 批准号: 23659818
• 负责人: SOTOZONO Chie
• 金额: $ 2.33万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659818/
• 关键词: アミノ酸; 角膜; 上皮細胞; タイトジャンクション; 涙液; ヒト角膜上皮細胞; アルギニン; グルタミン酸; グルタミン; アラニン; アスパラギン酸

We examined the change of cell growth, wound healing, and barrier function of cultivated human corneal epithelial cells using specific culture medium including or deleting 1-23 amino acids. The proliferation of cultivated human corneal epithelial cells was inhibited by deleting Arg, Glu, Asn or Ala from the culture medium containing 23 amino acids (FULL medium). Arg deficit from culture medium also inhibited wound healing at the vitro wound-healing model. Transepithelial electrical resistance (TER) was high in FULL medium, and low in ZERO medium, indicating amino acids increase cell-cell adhesion. In an Arg deficit culture medium, TER dramatically decreased. In the Glu deficit culture medium and the Asn deficit culture medium, it also decreased.

我们研究了培养的人角膜上皮细胞的细胞生长，伤口愈合和屏障功能的变化，使用特定的培养基包括或删除1-23个氨基酸。从含有23种氨基酸的培养基（FULL培养基）中去除Arg、Glu、Asn或Ala可抑制培养的人角膜上皮细胞的增殖。在体外伤口愈合模型中，培养基中的精氨酸缺陷也抑制伤口愈合。跨上皮电阻（TER）在FULL培养基中较高，在ZERO培养基中较低，表明氨基酸增加细胞-细胞粘附。在Arg缺陷培养基中，TER显著降低。在Glu缺乏培养基和Asn缺乏培养基中，也有所降低。

项目成果

Etiologic Features of Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) with Ocular Involvement.

史蒂文斯-约翰逊综合征 (SJS) 和累及眼部的中毒性表皮坏死松解症 (TEN) 的病因学特征。

• 发表时间: 2012
• 作者: Sotozono C;Ueta M;Kinoshita S;Kitami A;Iijima M;Aihara M;Ikezawa Z;Kano Y;Shiohara T;Shirakata Y;Sakabayashi S;Matsubara Y;Hashimoto K
• 通讯作者: Hashimoto K

Stevens-Johnson症候群の病態と治療

史蒂文斯-约翰逊综合征的病理学和治疗

• 发表时间: 2011
• 作者: Sotozono C;Ueta M;Kinoshita S;Kitami A;Iijima M;Aihara M;Ikezawa Z;Kano Y;Shiohara T;Shirakata Y;Sakabayashi S;Matsubara Y;Hashimoto K;外園千恵;外園千恵;外園千恵
• 通讯作者: 外園千恵

Stevens-Johnson 症候群、中毒性表皮壊死症（TEN）型薬疹と眼障害

史蒂文斯-约翰逊综合征、中毒性表皮坏死松解症 (TEN) 型药疹和眼部疾病

• 发表时间: 2011
• 作者: Sotozono C;Ueta M;Kinoshita S;Kitami A;Iijima M;Aihara M;Ikezawa Z;Kano Y;Shiohara T;Shirakata Y;Sakabayashi S;Matsubara Y;Hashimoto K;外園千恵;外園千恵;外園千恵;外園千恵
• 通讯作者: 外園千恵

Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry

• DOI: 10.1016/j.ajo.2010.11.003
• 发表时间: 2011-05-01
• 期刊: AMERICAN JOURNAL OF OPHTHALMOLOGY
• 影响因子: 4.2
• 作者: Nakatsukasa, Mina;Sotozono, Chie;Kinoshita, Shigeru
• 通讯作者: Kinoshita, Shigeru

Etiologic Features of Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) with Ocular Involvement

史蒂文斯-约翰逊综合征 (SJS) 和眼部受累的中毒性表皮坏死松解症 (TEN) 的病因学特征

• 发表时间: 2012
• 作者: Sotozono C;Ueta M;Kinoshita S;Kitami A;Iijima M;Aihara M;Ikezawa Z;Kano Y;Shiohara T;Shirakata Y;Sakabayashi S;Matsubara Y;Hashimoto K
• 通讯作者: Hashimoto K