The effects of the amino acid profile in tear fluid on ocular surface homeostasis and disease condition

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We examined the change of cell growth, wound healing, and barrier function of cultivated human corneal epithelial cells using specific culture medium including or deleting 1-23 amino acids. The proliferation of cultivated human corneal epithelial cells was inhibited by deleting Arg, Glu, Asn or Ala from the culture medium containing 23 amino acids (FULL medium). Arg deficit from culture medium also inhibited wound healing at the vitro wound-healing model. Transepithelial electrical resistance (TER) was high in FULL medium, and low in ZERO medium, indicating amino acids increase cell-cell adhesion. In an Arg deficit culture medium, TER dramatically decreased. In the Glu deficit culture medium and the Asn deficit culture medium, it also decreased.