Molecular and cellular mechanisms of DCX family proteins in brain development

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Doublecortin (Dcx) and Doublecortin-like kinases (Dclk1, Dclk2) function cooperatively in cortical layer formation and axon and dendrite formation that is required for the proper neural circuit formation. DCX family proteins deficiency is associated with epilepsy.In this project, we identified a novel target of DCLK1, MAP7D1. Depletion of MAP7D1 in cortical layer 2/3 pyramidal cells resulted in impaired axon elongation in the corpus callosum. Overexpression of unphosphorylated mutant MAP7D1, but not wild-type MAP7D1, also impaired callosal axon elongation. These results suggested that phosphorylation of MAP7D1 by DCLK1 regulate axon elongation in neurons.