Role of prosaposin in the mouse embryogenesis

prosaposin在小鼠胚胎发生中的作用

• 批准号: 23700515
• 负责人: YONESHIGE Azusa
• 金额: $ 2万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700515/
• 关键词: 胚発生; プロサポシン; サポシン

Prosaposin (PSAP) is the precursor protein of saposins (SAPs) which are required for the lysosomal hydrolysis of sphingolipids. Although PSAP itselfis known to be secreted into several body fluids, its physiological function is not well understood. PSAP deficient mice (Psap-/-) are born at the much lower frequency suggesting the function of PSAP during the mouse embryogenesis. In this study, we investigated the temporal and spatial expression of PSAP in the wild-type mouse embryo and the phenotype of Psap-/-embryo. Protein levels of PSAP in the mouse embryonic tissues were low at embryonic day (E) 6.5 and increased at E7.5 to 9.5. Immunohistochemical analyses showed high expressions of PSAP in decidua at E7.5 to 9.5 and in spongiotrophoblastic layer of placenta at E10.5. SAPs were highly expressed in the large lysosomes of visceral endoderm of yolk sac. Histopathological examination of Psap-/-embryos indicated the developmental abnormality as early as at around E7.5. Shrunken cells were observed in decidua. The number and the size of large lysosome in visceral endoderm of yolk sac were decreased. These lethal phenotypes of Psap-/-embryos were rescued by maternal overexpression of PSAP. Our findings suggest that PSAP and SAPs play important roles during the early stage of mouse pregnancy, at the site of decidua, yolk sac and placenta.

鞘脂激活蛋白原（PSAP）是鞘脂激活蛋白（SAP）的前体蛋白，其是鞘脂的溶酶体水解所必需的。虽然已知PSAP本身可分泌到多种体液中，但其生理功能尚不清楚。PSAP缺陷型小鼠（Psap-/-）的出生频率低得多，表明PSAP在小鼠胚胎发育过程中的功能。在这项研究中，我们研究了PSAP在野生型小鼠胚胎中的时空表达和Psap-/-胚胎的表型。小鼠胚胎组织中PSAP的蛋白水平在胚胎日（E）6.5时较低，并在E7.5至9.5时增加。免疫组化分析显示PSAP在E7.5至9.5的蜕膜和E10.5的胎盘海绵滋养层中高表达。SAP在卵黄囊内脏内胚层的大溶酶体中高度表达。Psap-/-胚胎的组织学检查表明早在E7.5左右就出现发育异常。蜕膜细胞皱缩。卵黄囊脏内胚层大溶酶体数量减少，体积缩小。PSAP-/-胚胎的这些致死表型被PSAP的母体过表达所拯救。我们的研究结果表明，PSAP和SAP在小鼠妊娠早期，在蜕膜，卵黄囊和胎盘的位置发挥重要作用。

项目成果

ライソゾーム病－最新の病態、診断、治療の進歩：サポシン欠損症

溶酶体疾病——病理学、诊断和治疗的最新进展：saposin缺乏症

• 发表时间: 2011
• 作者: 松田純子;米重あづさ
• 通讯作者: 米重あづさ

Chemoenzymatic synthesis of hydrophobic glycoprotein: synthesis of saposin C carrying complex-type carbohydrate.

• DOI: 10.1021/jo3010155
• 发表时间: 2012-07
• 期刊: The Journal of organic chemistry
• 作者: H. Hojo;Hiromasa Tanaka;M. Hagiwara;Yuya Asahina;A. Ueki;H. Katayama;Y. Nakahara;Azusa Yoneshige;J. Matsuda;Yukishige Ito;Y. Nakahara

プロサポシン強発現マウス胚組織の表現型解析

强表达prosaposin的小鼠胚胎组织的表型分析

• 发表时间: 2012
• 作者: 武藤真長;米重あづさ;昼沢良介;吉村真一;松田純子
• 通讯作者: 松田純子

The effects of chemically synthesized saposin C onglucosylceramide-β-glucosidase.

化学合成的皂苷 C 对葡萄糖神经酰胺-β-葡萄糖苷酶的影响。

• DOI: 10.1016/j.clinbiochem.2015.06.004
• 发表时间: 2015
• 期刊: Clinical Biochemistry
• 影响因子: 2.8
• 作者: Yoneshige A;Muto M;Watanabe T;Hojo H;Matsuda J.
• 通讯作者: Matsuda J.

クラッベ病モデルマウスの免疫系組織の病態解析

克拉伯病模型小鼠免疫系统组织的病理分析

• 发表时间: 2012
• 作者: 佐藤正宏;赤坂恵理;斉藤一誠;大塚正人;渡部聡;吉川彩
• 通讯作者: 吉川彩