Possible functions of the permeability barrier of the nuclear envelope.

核膜渗透屏障的可能功能。

• 批准号: 24790103
• 负责人: SHIMOZONO Satoshi
• 金额: $ 2.33万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790103/
• 关键词: 核膜; 蛍光タンパク質; 拡散; 核膜の透過性

I studied the phenomenon of the high nuclear envelope (NE) permeability observed in the mouse fibroblast, Swiss 3T3 cell line. In this research, I obtained the following results by live cell imaging techniques using fluorescent proteins as probes for the permeability. First, the high NE permeability was not persistent but transient. Second, the calcium ion concentration in the cytoplasm, which is reported to affect the NE permeability, was not the cause of the high NE observed in Swiss3T3 cells. Third, this phenomenon was not specific only to the Swiss3T3 cell line but could be general because the high NE permeability was also observed in the NIH3T3 cell line and the HT1080 cell line.

我研究了在小鼠成纤维细胞瑞士3T3细胞系中观察到的高核膜（NE）通透性现象。在本研究中，我使用荧光蛋白作为通透性探针，通过活细胞成像技术获得了以下结果：首先，高NE导率不是持续的，而是短暂的。其次，细胞质中的钙离子浓度（据报道会影响NE通透性）并不是Swiss3T3细胞高NE的原因。第三，这一现象并非Swiss3T3细胞系所特有，而可能具有普遍性，因为在NIH3T3细胞系和HT1080细胞系中也观察到高NE通透性。