Multilayered Cell Assembling Technology Using Dielectrophoresis and Construction of Multilayered Skin Tissue Array

介电泳多层细胞组装技术及多层皮肤组织阵列的构建

• 批准号: 24710145
• 负责人: MIYATA Shogo
• 金额: $ 3万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24710145/
• 关键词: 多階層組織; 真皮; 表皮; 誘電泳動; 細胞チップ; 細胞アセンブリ; 多層皮膚組織エレメント; 皮膚

The objective of this study was to reconstruct multilayered structure of micro organization using dielectrophoresis (DEP). DEP is a phenomenon in which a force is generated on a cell or particle when it is subjected to a non-uniform electric field. In this study, alginate-collagen micro-beads were developed for dielectrophoresis experiments and cell adhesive scaffold. Dermal and epi-dermal skin cells were assembled by DEP force to cover the beads and to construct multilayered structure. After the cell assembly, the cell-accumulated beads were cultured in vitro to reconstruct maultilayered skin tissue. Finally, the cell viability and proliferation of the cultured constructs were maintained during 7 days culture.

本研究的目的是利用介电泳技术重建微组织的多层结构。DEP是当细胞或颗粒受到非均匀电场时在其上产生力的现象。本研究以海藻酸钠-胶原微球为材料，进行介电电泳实验和细胞黏附支架的研究。通过DEP力组装真皮和表皮皮肤细胞以覆盖珠粒并构建多层结构。细胞组装后，细胞聚集珠在体外培养，以重建多层皮肤组织。最后，在7天培养期间维持培养的构建体的细胞活力和增殖。

项目成果

誘電泳動を用いたラベルフリー＆ダメージレス細胞ソーティング

使用介电泳进行无标记和无损伤的细胞分选

• 发表时间: 2013
• 作者: Minami Yamashita;shogo Miyata;Shinya Takeuchi;Hajime Inoue;宮田昌悟
• 通讯作者: 宮田昌悟

High Throughput Cell Sorting Device Using Dielectrophoresis and Fluid-Induced Shear Force, Engineering in Medicine and Biology Society (EMBC)

使用介电泳和流体诱导剪切力的高通量细胞分选装置，医学与生物学工程学会 (EMBC)

• 发表时间: 2013
• 作者: Yuwa Takahashi;Shinya Takeuchi;Shogo Miyata
• 通讯作者: Shogo Miyata

Separation method of blood constituents using dielectrophoresis and flow-induced shear force, Engineering in Medicine and Biology Society (EMBC)

使用介电泳和流动诱导剪切力的血液成分分离方法，医学与生物学工程学会 (EMBC)

• 发表时间: 2013
• 作者: Minami Yamashita;shogo Miyata;Shinya Takeuchi;Hajime Inoue
• 通讯作者: Hajime Inoue

誘電泳動を応用した多階層細胞アセンブリ技術と皮膚組織再構築への展開

介电泳多级细胞组装技术及其在皮肤组织重建中的应用

• 发表时间: 2013
• 作者: Minami Yamashita;shogo Miyata;Shinya Takeuchi;Hajime Inoue;宮田昌悟;宮田昌悟
• 通讯作者: 宮田昌悟