Multilayered Cell Assembling Technology Using Dielectrophoresis and Construction of Multilayered Skin Tissue Array

本研究的目的是利用介电泳法(DEP)重建多层微组织结构。DEP是一种当细胞或粒子受到非均匀电场时产生力的现象。本研究制备了海藻酸盐-胶原微球，用于介电实验和细胞黏附支架。真皮和真皮细胞在DEP力的作用下组装在珠子上，形成多层结构。细胞组装后，体外培养细胞聚集珠，构建成多层皮肤组织。最后，在7天的培养过程中，培养的构建体保持了细胞的活性和增殖。

The objective of this study was to reconstruct multilayered structure of micro organization using dielectrophoresis (DEP). DEP is a phenomenon in which a force is generated on a cell or particle when it is subjected to a non-uniform electric field. In this study, alginate-collagen micro-beads were developed for dielectrophoresis experiments and cell adhesive scaffold. Dermal and epi-dermal skin cells were assembled by DEP force to cover the beads and to construct multilayered structure. After the cell assembly, the cell-accumulated beads were cultured in vitro to reconstruct maultilayered skin tissue. Finally, the cell viability and proliferation of the cultured constructs were maintained during 7 days culture.