Multilayered Cell Assembling Technology Using Dielectrophoresis and Construction of Multilayered Skin Tissue Array

本研究的目的是利用介电电泳（DEP）重建微组织的多层结构。DEP是一种现象，当细胞或粒子受到非均匀电场时，会对其产生力。本研究制备了海藻酸胶原微球，用于电泳实验和细胞黏附支架。在DEP力作用下，真皮和真皮外表皮细胞聚集在一起，形成多层结构。细胞组装完成后，将细胞积累的微球体外培养，重建多层皮肤组织。最后，在7天的培养过程中，培养的构建体的细胞活力和增殖能力保持不变。

The objective of this study was to reconstruct multilayered structure of micro organization using dielectrophoresis (DEP). DEP is a phenomenon in which a force is generated on a cell or particle when it is subjected to a non-uniform electric field. In this study, alginate-collagen micro-beads were developed for dielectrophoresis experiments and cell adhesive scaffold. Dermal and epi-dermal skin cells were assembled by DEP force to cover the beads and to construct multilayered structure. After the cell assembly, the cell-accumulated beads were cultured in vitro to reconstruct maultilayered skin tissue. Finally, the cell viability and proliferation of the cultured constructs were maintained during 7 days culture.