Funktion Eisen-transportierender CDF-Proteine in magnetotaktischen Bakterien und E. coli

趋磁细菌和大肠杆菌中铁转运 CDF 蛋白的功能

• 批准号: 5443719
• 负责人: Professor Dr. Dietrich H. Nies
• 金额: --
• 依托单位: Institutsbereich Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5443719?language=en
• 关键词: Funktion; Eisen; transportierender; CDF; Proteine

A specific subset of proteins that belong to the cation diffusion facilitator (CDF) family of membrane-bound transporters very frequently occurs in prokaryotes, but the physiological function of these proteins has remained obscure until recently. Data obtained for members of that family, Escherichia coli (FieF, former name Yiip) and Magnetospirillum gryphiswaldense (MamB, MamM), indicate that these proteins might transport Fe(II) and be part of the cellular iron homeostasis system in bacteria. E.coli and FieF are easy to manipulate by the methods of molecular genetics. MamB and MamM, on the orther hand are putative iron transporters with unusual properties since magnetosome biomineralization requires the transport of vast amounts of iron. In addition, M. gryphiswaldense has recently become amenable by genetic techniques, and magnetosome formation is a unique and conspicuous phenomenon. Therefore, the groups in Halle and Bremen joined forces to engage into the highly competitive field of iron transport by CDF proteins in an interlinked back-to-back approach: The physiological functions and biochemical mechanism of FieF and CDF-like Mam-proteins involved in magnetosome biosynthesis will be studied using a highly intergrated genetic, biochemical and physological approach. Ultimately, we not only expect to unveil the metabolic relevance of the specified proteins in iron homeostasis and magnetosome formation, respectively, but we will be able to assign a function to the whole family of so far uncharacterized CDF3 proteins.

属于膜结合转运蛋白的阳离子扩散促进剂（CDF）家族的蛋白质的特定子集非常频繁地发生在原核生物中，但是这些蛋白质的生理功能直到最近仍然不清楚。该家族成员获得的数据，大肠埃希菌 (FieF，前名Yiip）和Magnetoeplillum gryphiswaldense（MamB，MamM）的研究表明，这些蛋白质可能转运Fe（II），并且是细胞铁稳态系统的一部分， 细菌大肠杆菌和FieF易于通过分子遗传学方法操作。另一方面，MamB和MamM是 自磁小体以来具有不寻常特性的推定铁转运蛋白 生物矿化需要运输大量的铁。此外，M. gryphiswaldense最近已经变得适合于遗传技术，磁小体形成是一种独特而显著的现象。因此，哈雷和不莱梅的团体联合起来， CDF蛋白在相互连接的背靠背方法中铁转运的高度竞争领域：FieF和CDF样Mam蛋白的生理功能和生化机制 将使用高度整合的遗传学、生物化学和生理学方法来研究参与磁小体生物合成的基因。最终，我们不仅期望揭示特定蛋白质在铁稳态中的代谢相关性， 和磁小体的形成，但我们将 能够将功能分配给迄今为止未表征的CDF 3蛋白的整个家族。