Molekulare Funktion von Proteinen der cation diffusion facilitator Proteinfamilie untersucht am Beispiel des CzcD- und des Dme-Proteins aus Wauterisia (Ralstonia) metallidurans

ChecD蛋白是阳离子扩散促进剂(CDF)蛋白家族中第一个被描述的成员。CDF蛋白存在于生命的各个领域，包括人类，是锌(以及钴、镉，可能还有铁)动态平衡的重要因素。我们建议的目标是：(I)与Hartmut Michel(MPI BiPhysik，法兰克福)合作获得ChucD蛋白的晶体结构；(Ii)与Christopher Rensing(亚利桑那州图森)合作，了解CDF蛋白在金黄色葡萄球菌和大肠杆菌中的生理功能；(Iii)开发CDF蛋白的生化测试系统，例如蛋白脂体模型(Halle)和大肠杆菌内向外囊泡(Tucson)；以及(Iv)通过定点诱变的方法追踪重金属阳离子从细胞膜的一面到另一面的方式。

The CzcD protein is the first described member of the cation diffusion facilitator (CDF) protein family. CDF proteins occur in all domains of life, including man, and are important factors for zinc (and cobalt, cadmium, maybe iron) homoeostasis. The goals of our proposal are (i) to obtain the crystal structure of the CzcD protein in co-operation with Hartmut Michel (MPI Biophysik, Frankfurt), (ii) to understand the physiological function of CDF proteins in Ralstonia metallidurans and Escherichia coli in co-operation with Christopher Rensing (Tucson, Arizona), (iii) to develop biochemical test systems for CDF proteins, e.g. the proteoliposome model (Halle) and E. coli inside-out vesicles (Tucson) and (iv) to track down the way of a heavy metal cation from one face of the cytoplasmic membrane to the other face by means of site directed mutagenesis.