Molekulare Funktion von Proteinen der cation diffusion facilitator Proteinfamilie untersucht am Beispiel des CzcD- und des Dme-Proteins aus Wauterisia (Ralstonia) metallidurans

CzcD蛋白是阳离子扩散促进剂（CDF）蛋白家族中第一个被描述的成员。CDF蛋白存在于包括人类在内的所有生命领域，是锌（以及钴、镉，可能还有铁）平衡的重要因素。我们提案的目标是(i)与Hartmut Michel （MPI Biophysik, Frankfurt）合作获得CzcD蛋白的晶体结构，（ii）与Christopher Rensing （Tucson, Arizona）合作了解CDF蛋白在金属拉尔斯顿菌和大肠杆菌中的生理功能，（iii）开发CDF蛋白的生化测试系统。例如，蛋白质脂质体模型（Halle）和大肠杆菌内外囊泡（Tucson），以及（iv）通过位点定向诱变方法追踪重金属阳离子从细胞质膜的一面到另一面的路径。

The CzcD protein is the first described member of the cation diffusion facilitator (CDF) protein family. CDF proteins occur in all domains of life, including man, and are important factors for zinc (and cobalt, cadmium, maybe iron) homoeostasis. The goals of our proposal are (i) to obtain the crystal structure of the CzcD protein in co-operation with Hartmut Michel (MPI Biophysik, Frankfurt), (ii) to understand the physiological function of CDF proteins in Ralstonia metallidurans and Escherichia coli in co-operation with Christopher Rensing (Tucson, Arizona), (iii) to develop biochemical test systems for CDF proteins, e.g. the proteoliposome model (Halle) and E. coli inside-out vesicles (Tucson) and (iv) to track down the way of a heavy metal cation from one face of the cytoplasmic membrane to the other face by means of site directed mutagenesis.