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Mechanismus und Kontrolle der Zink-Aufnahme im Modellorganismus Cupriavidus metallidurans CH34

模式生物 Cupriavidus metallidurans CH34 锌摄取的机制和控制

基本信息

批准号：
67192400
负责人：
Professor Dr. Dietrich H. Nies
金额：
--
依托单位：
Institutsbereich Mikrobiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/67192400?language=en
关键词：
Mechanismus und Kontrolle der Zink

项目摘要

Sophisticated cellular biochemistry needs metals as cofactors. About 40% of all enzymes have metal cofactors. It is an interesting question how the correct metal is allocated to the right protein, which is especially complicated for divalent metal cations that have a similar ionic diameter and volume-tocharge ratios, and compete with each other for the metal binding sites in enzymes. The β−proteobacterium Cupriavidus metallidurans strain CH34 is able to keep its metal homeostasis under a variety of adverse conditions and became a prokaryotic model organism to study such a process. Most of its’ efflux systems, required to remove surplus metals from the cells, are known. In the first part of the proposal, many uptake systems for zinc were identified. We would like to continue by pursuing the following goals: (i) the inventory of zinc importers in strain CH34 shall be completed, (ii) the metal transport capacities of the importers from the metal-resistant strain CH34 shall be compared to those from E. coli, and (iii) the regulators of gene expression of the transport systems are to be identified. Goal number four stems from the unexpected observation that zinc starvation leads to disappearance of CzcA, the major component of the efflux protein complex CzcCBA. We would like to identify the mechanism behind this process.

复杂的细胞生物化学需要金属作为辅助因子。大约40%的酶都有金属辅助因子。如何将正确的金属分配给正确的蛋白质是一个有趣的问题，这对于具有相似离子直径和体积电荷比的二价金属阳离子来说尤其复杂，并且它们在酶中相互竞争金属结合位点。β -变形菌Cupriavidus metallidurans菌株CH34能够在各种不利条件下保持其金属稳态，成为研究这一过程的原核模式生物。它的大部分外排系统都是已知的，这些系统需要从细胞中去除多余的金属。在提案的第一部分中，确定了许多锌的吸收系统。我们希望继续追求以下目标：(i)完成菌株CH34中锌进口商的清单，（ii）将耐金属菌株CH34的进口商与大肠杆菌的金属运输能力进行比较，以及（iii）确定运输系统基因表达的调节因子。第四个目标源于一个意想不到的观察，即锌缺乏导致CzcA的消失，CzcA是外排蛋白复合物CzcCBA的主要成分。我们想要确定这一过程背后的机制。