Development of a new high-throughput TCR-antigen pair identification method toward cancer immune therapy

开发用于癌症免疫治疗的新型高通量TCR抗原对鉴定方法

• 批准号: 17F17389
• 负责人: 城口 克之
• 金额: $ 1.47万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2017
• 资助国家: 日本
• 起止时间: 2017-10-13 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17389/
• 关键词: イメージング; ピッキング; 細胞相互作用; Ｔ細胞; TCR; T cell; sequencing; cell-cell interaction; microscopy

We have been building up a microscopic platform for scanning the real-time cell behaviors, such as cell movement and T cell-target cell interactions. For high-throughput scanning of these behaviors, we have checked that scanning rate of the current system, and found that the speed of stage movement may be enough for the observation of T cell activities. However, in order to link the microscope system to following steps, we found that real-time calculation based on obtained cell images was required. Then, we investigated specifications of a software based on our actual measurements for T cell-target cell interactions. This helps one to combine imaging and following genomic analyses using our cell picking system.

我们一直在建立一个微观平台，用于扫描实时细胞行为，例如细胞运动和T细胞靶细胞相互作用。 对于这些行为的高通量扫描，我们检查了当前系统的扫描速率，并发现阶段运动的速度可能足以观察到T细胞活动。 但是，为了将显微镜系统与以下步骤联系起来，我们发现需要基于获得的单元格图像的实时计算。 然后，我们根据我们针对T细胞目标细胞相互作用的实际测量进行了研究软件的规格。 这有助于使用我们的细胞拾取系统结合成像并进行基因组分析。

项目成果

A novel high-throughput method for quantification of microbiota distribution at the single cell level

一种在单细胞水平上定量微生物群分布的新型高通量方法

• 发表时间: 2017
• 作者: Jianshi Jin;Tadashi Takeuchi;Eiji Miyauchi;Hiroshi Ohno;Katsuyuki Shiroguchi
• 通讯作者: Katsuyuki Shiroguchi

An applicational study of a novel developed method BarBIQ: analysis of microbiota in different locations of a murine cecum & colon

一种新开发的方法 BarBIQ 的应用研究：小鼠盲肠不同位置的微生物群分析

• 发表时间: 2018
• 作者: Janshi Jin;Reiko Yamamoto;Tadashi Takeuchi;Eiji Miyauchi;Hiroshi Ohno;Katsuyuki Shiroguchi
• 通讯作者: Katsuyuki Shiroguchi

A novel high-throughput method for bacteria counting and de-novo 16S rRNA sequence identification with single-base resolution

一种新型高通量单碱基分辨率细菌计数和从头 16S rRNA 序列鉴定方法

• 发表时间: 2017
• 作者: Jianshi Jin1;Kaori Fukuhara;Tadashi Takeuchi;Eiji Miyauchi;Hiroshi Ohno;Katsuyuki Shiroguchi
• 通讯作者: Katsuyuki Shiroguchi

Probing Taxa-Specific Bacterial Coordinational Distribution in Intestinal Microbiota by Super-accurate Method BarBIQ

通过超精确方法探索肠道微生物群中特定分类群的细菌协调分布 BarBIQ

• 发表时间: 2019
• 作者: J. Jin;R. Yamamoto;T. Takeuchi;E. Miyauchi;H. Ohno;K. Shiroguchi
• 通讯作者: K. Shiroguchi