Enhancement of hypoxic tolerance, and clarifying of oxygen acquisition mechanism of root of millet and buckwheat under hypoxia

增强耐缺氧能力并阐明低氧条件下小米和荞麦根部的吸氧机制

• 批准号: 22K05602
• 负责人: 松浦 朝奈
• 金额: $ 2.66万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05602/
• 关键词: 耐湿性; 雑穀; ソバ; 根; 成長; 生理生態; 低酸素耐性; 破生通気組織

雑穀3種を通気した対照区と窒素を通気後に静置した低酸素区の培養液にエセフォン濃度を添加した処理区を加えて2週間栽培した．対照区にエセフォンを1mg/ml添加すると，すべての雑穀で個体成長速度に有意差はなかったが，ヒエでは増加する傾向を示し，テフでは変化せず，アワでは低下する傾向を示した．低酸素区では，テフの個体成長速度は有意に減少した．一方，ヒエとアワでは有意差はなかったが，増加する傾向を示した．ヒエとテフの根のTTＣ反応は，すべての処理区で同様に赤く呈色したが，アワの低酸素区では最も薄く，エセフォンを添加した場合には低酸素区より赤く呈色した．ヒエはエセフォンの添加に関わらず対照区と低酸素区で根端2cmから，テフは根端1cmから通気組織が観察された．アワでは，対照区ではエセフォンの添加に関わらず通気組織は観察されなかった．低酸素区でも通気組織は観察されなかったが，エセフォンの添加によってわずかに通気組織が観察された．砂土を充填した塩化ビニル製の円筒にソバ５品種の種子を播種し，播種後15日目（久木野，常陸秋そば，九系55）と22日目（宿根ソバ，FG）に溶存酸素濃度を1mg/L以下とする培養液で円筒表面まで浸水した低酸素区と円筒底部から5cmまで培養液に浸漬した対照区を設けて20日間栽培した．低酸素処理によって個体成長速度は13～40％に著しく低下した．個体成長速度から算出したストレス感受性指数は，FG＞久木野＝宿根ソバ＝常陸秋そば＝九系55の順となった．FGと宿根ソバの葉面積／根の乾物重比は低酸素処理によって変化しなかったが，そのほかのソバでは増加する傾向を示した．すべてのソバの根の内部に通気組織が確認されなかった．また，すべてのソバの根の下皮にはスベリンの蓄積が観察された．根の画像から，FGの低酸素区の根系は，他のそばに比べて長さ10cm以下の短い根が多かった．

The temperature of the incubation solution in the low-acid area is not stable. The temperature of the culture solution in the low-acid area is increased. The temperature is added in the temperature zone. The temperature of the 1mg/ml in the light area is increased, and the growth rate of the body is intentionally different. The growth rate of the plant is intentionally different from the temperature, and the temperature increases the temperature to indicate the temperature, and the temperature increases the temperature. In the low-acid area, the growth rate is intentionally lower than that in the low-acid area. On the other hand, the growth rate of the low-acid area is lower than that in the low-acid area. On the other hand, the growth rate of the low-acid area is lower than that in the low-acid area, and the growth rate of the low-acid area is lower than that in the low-acid area. on the other hand, the growth rate of the low-acid area is the highest in the low-acid area. In the low-acid area, the color is displayed in the low-acid area. The low-acid area is in the low-acid area. In the low-acid area, there is a 2cm in the root end of the low-acid area, and the 1cm in the root end. In the light area, the general information organization is responsible for the monitoring of environmental pollution. In the low-acid area, the general organization is responsible for monitoring the environmental pollution, and the low-acid area is responsible for the monitoring of the environmental pollution. The sandy soil filling is used to improve the quality of the food, and it will be broadcast on the 15th day after the broadcast (Nagi Ye, autumn festival). Nine series 55) 22 orders (perennial roots) FG) the temperature of soluble acid is below 1mg/L, the surface of the tank is immersed in water, the low acid zone, the bottom of the tube, the immersion zone, the illuminated area, the temperature, the temperature, the pH, the acid content, the FG > Nagi Nakagi = Nine-line 55-year-old Akiyama, FG-55, Nine-line, 55-year-old, five-year-old, four-year-old, five-year-old, five-year-old, four-year-old, five-year-old, five-year-old, four-year-old, five-year-old, old, old, In this case, you can see the image of the root, the root of the FG, the root of the low-acid area, the root of the root, the root.