Impact of Land-use on Biogeographical Diversity of Soil Prokaryotes and their Functional Links to Atmospheric Chemistry

土地利用对土壤原核生物生物地理多样性的影响及其与大气化学的功能联系

• 批准号: 61396413
• 负责人: Professor Dr. Harold L. Drake
• 金额: --
• 依托单位: Institut für Mikrobiologie (ifmb)
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/61396413?language=en
• 关键词: Impact; Land; use; Biogeographical; Diversity

The functional biodiversity and differential regulation of subpopulations of prokaryotic biomes associated with atmospheric chemistry will be investigated. The study will address the question “What determines what one gets?” with a comprehensive, integrative experimental design that will evaluate soils under contrasting land-use. The objectives are to (a) assess denitrifier and methylotroph diversities by complementary cultivation and functional gene analyses, (b) determine the effect of land-use on activities of denitrifiers and methylotrophs, (c) identify active denitrifiers and methylotrophs by mRNA analyses and stable isotope probing, (d) identify novel genotypes, (e) resolve temporal/spatial variations in community structures and develop a diagnostic functional gene microarray, and (f) establish a functional gene-based ‘ecotype concept’ for denitrifiers and methylotrophs. An additional outcome will be the improvement of methods for the assessment of these functional groups. By correlating data on functional diversity and activity with the database on in situ soil parameters of the biodiversity exploratories, this work will also increase our understanding of how prokaryotic diversity impacts on ecosystem function and provide insight into factors that might drive prokaryotic speciation.

将研究与大气化学相关的原核生物生物群落亚群的功能性生物多样性和差异调节。这项研究将解决一个问题：“什么决定了什么？”采用全面的，集成的实验设计，可以评估土壤中的土壤。目的是（a）通过互补的培养和功能基因分析来评估非替代剂和甲基营养的多样性，（b）确定土地利用对定位剂和甲基营养物的活性的影响，（c）通过mRNA分析和稳定的概率（disoties）识别（distable proiniation），（c）通过（distable）识别出活性的定位剂和甲基营养物的临时概率（distable）（disotiation）（distable）（disotiation）（distable）（distable）（distable）（d）的新颖性（d）（d）（d）（d）（d）（d）（d）在社区结构并开发诊断功能基因微阵列中，（f）建立一个基于功能基因的“生态型概念”，用于分析剂和甲基营养物。另一个结果将是改善评估这些官能团的方法。通过将功能多样性和活性的数据与生物多样性探索的原位土壤参数的数据库相关联，这项工作还将增加我们对原核生物多样性对生态系统功能的影响的理解，并提供对可能驱动原核规范的因素的洞察力。