Impact of Land-use on Biogeographical Diversity of Soil Prokaryotes and their Functional Links to Atmospheric Chemistry

与大气化学相关的原核生物群落亚群的功能生物多样性和差异调节将被调查。这项研究将解决“什么决定一个人得到什么？”这个问题。具有全面、综合的实验设计，将在不同的土地使用情况下评估土壤。目标是：(A)通过互补培养和功能基因分析来评估反硝化细菌和甲基营养细菌的多样性；(B)确定土地利用对反硝化细菌和甲基营养细菌活性的影响；(C)通过mRNA分析和稳定同位素探测来鉴定活跃的反硝化细菌和甲基营养细菌；(D)鉴定新的基因类型；(E)解决群落结构中的时空变化并开发诊断功能基因芯片；以及(F)建立基于功能基因的反硝化细菌和甲基营养细菌的生态型概念。另一个结果将是改进评估这些官能团的方法。通过将功能多样性和活性的数据与生物多样性探索实验室的土壤原位参数数据库相关联，这项工作还将增加我们对原核生物多样性如何影响生态系统功能的理解，并为可能推动原核生物物种形成的因素提供洞察。

The functional biodiversity and differential regulation of subpopulations of prokaryotic biomes associated with atmospheric chemistry will be investigated. The study will address the question “What determines what one gets?” with a comprehensive, integrative experimental design that will evaluate soils under contrasting land-use. The objectives are to (a) assess denitrifier and methylotroph diversities by complementary cultivation and functional gene analyses, (b) determine the effect of land-use on activities of denitrifiers and methylotrophs, (c) identify active denitrifiers and methylotrophs by mRNA analyses and stable isotope probing, (d) identify novel genotypes, (e) resolve temporal/spatial variations in community structures and develop a diagnostic functional gene microarray, and (f) establish a functional gene-based ‘ecotype concept’ for denitrifiers and methylotrophs. An additional outcome will be the improvement of methods for the assessment of these functional groups. By correlating data on functional diversity and activity with the database on in situ soil parameters of the biodiversity exploratories, this work will also increase our understanding of how prokaryotic diversity impacts on ecosystem function and provide insight into factors that might drive prokaryotic speciation.