Identification of genes involved in the symbiotic interactions in the lichen Xanthoria parietina

地衣 Xanthoria parietina 中参与共生相互作用的基因的鉴定

• 批准号: 73193622
• 负责人: Dr. Andreas Beck
• 金额: --
• 依托单位: Botanische Staatssammlung München
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/73193622?language=en
• 关键词: Identification; genes; involved; symbiotic; interactions

Lichens are composed of at least two different organisms, an alga (photobiont) and a fungus (mycobiont). These form an often highly differentiated structure, the lichen thallus. Even if this interaction between the partaking symbionts is not exclusive, many mycobionts are rather selective in their photobiont choice, like, for example, the foliose lichen Xanthoria parietina. Both partners can be isolated into axenic cultures and cultivated separately in the laboratory. The first steps of the relichenisation can be induced by co-culturing these isolates. This offers the opportunity to study the molecular mechanisms underlying the establishment of lichen symbiosis. The symbionts exchange signals leading to close metabolic coupling (e.g. release of sugar alcohols by the alga and subsequent uptake and metabolism by the mycobiont) and finally responses to light, leading to an optimal arrangement of the algae in a lichen thallus to perform photosynthesis. Genes involved in the very early symbiotic interaction of the symbionts will be identified in order to gain first insight in the genetic basis of signal exchange in lichen symbiosis.

地衣至少由两种不同的生物体组成，一种是浮游生物（photobiont），另一种是真菌（mycobiont）。它们形成一种高度分化的结构，即地衣叶状体。即使共生体之间的这种相互作用不是排他性的，但许多真菌生物在选择光合生物时是相当有选择性的，例如叶状地衣Xanthoria parietina。这两种伴侣可以分离到无菌培养物中，并在实验室中单独培养。再地衣化的第一步可以通过共培养这些分离株来诱导。这为研究地衣共生的分子机制提供了机会。共生体交换信号，导致密切的代谢偶联（例如，糖醇的释放由真菌和随后的吸收和代谢），并最终响应于光，导致藻类在地衣叶状体中的最佳排列，以进行光合作用。在非常早期的共生相互作用的共生体中所涉及的基因将被确定，以获得在地衣共生信号交换的遗传基础的第一次洞察。