The composition and the pathological significance of C1q-binding immune complexes in MRL/lpr mice

MRL/lpr小鼠C1q结合免疫复合物的组成及其病理意义

• 批准号: 09671152
• 负责人: UWATOKO Shu
• 金额: $ 1.98万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671152/
• 关键词: systemic lupus erythematosus; MRL; lpr mice; immune complexes; autoantibodies; C1q; antiDNAantibodies; collagen-like region of C1q; 全身性エリテマトーテス; eprマウス; C1q; C1qコラーゲン部; lマウス

Our previous studies have shown that the majority of C1q-binding IgG in patients with systemic lupus erythematosus (SLE) is composed of autoantibodies to the collagen-like region of C1q. We also showed that C1q-binding IgG in MRL/l mice, in contrast to human SLE, is large-sized immune complexes containing antibodies to DNA.DNase I digestion of C1q-binding IgG failed to diminish C1q-binding activity, suggesting that other negatively-charged substances might be present as antigens in these C1q-binding immune complexes.This study was undertaken to characterize the antigens in C1q-binding immune complexes in MRL/l mice. Laminin and collagens were tested by ELISA, and glycosaminoglycans (hyaluronic acid, chondroitin sulfate, heparin, heparan sulfate) were examined by the effects of enzyme-digestion (hyaluronidase, chondroitinase, etc.) on C1q-binding activities of these immune complexes. In addition, the effects of phospholipases were also investigated for possible involvement of lipids in C1q-binding activity. No evidence, however, was obtained for the presence of these substances in the C1q-binding immune complexes.

我们以前的研究表明，在系统性红斑狼疮(SLE)患者中，C1q结合的免疫球蛋白大部分是由C1q胶原区的自身抗体组成的。我们还发现，与人类系统性红斑狼疮相比，MRL/L小鼠中C1q结合的免疫球蛋白是含有抗DNA抗体的大型免疫复合体。DNA酶I消化C1q结合的免疫球蛋白并不能降低C1q结合活性，这表明在这些C1q结合的免疫复合体中可能存在其他负电荷物质。层粘连蛋白和胶原蛋白检测采用酶联免疫吸附试验，糖胺多糖(透明质酸、硫酸软骨素、肝素、硫酸肝素)检测采用酶消化法(透明质酸酶、软骨素酶等)。这些免疫复合体的C1q结合活性。此外，还研究了磷脂酶对C1q结合活性的影响，以确定脂类对C1q结合活性的影响。然而，没有证据表明这些物质存在于C1q结合的免疫复合体中。

项目成果

上床 周: "看護のための基礎知識(林茂樹編)、泌尿器疾患(基礎知識、腎不全、全身疾患と腎不全)" 真興交易医書出版部, 61 (1997)

Shu Uetoko：“护理基础知识（林茂树编辑），泌尿系统疾病（基础知识，肾衰竭，全身疾病和肾衰竭）”新光贸易医学出版部，61（1997）

関 常司: "Mechanism of anion permeation in the basolateral membrane of isolated rabbit rental proximal tubule S3 segment." Am.J.Physiol.(Cell Physiolagy). 272. C837-C846 (1997)

Tsuneji Seki：“离体出租兔近端小管 S3 段基底外侧膜的阴离子渗透机制。”Am.J.Physiol.（细胞生理学）272.C837-C846（1997）。

Seki, G., Yamada, H., Taniguchi, S., Uwatoko, S., Suzuki, K., Kurokawa, K.: "Mechanism of anion permeaation in the basolateral membrane of isolated rabbit renal proximal tubule S3 segment." Am. J.Physiol. 272. C837-846 (1997)

Seki, G.、Yamada, H.、Taniguchi, S.、Uwatoko, S.、Suzuki, K.、Kurokawa, K.：“离体兔肾近端小管 S3 段基底外侧膜阴离子渗透机制”。

Okawa-Takatsuji, M., Aotsuka, S., Fujinami, M., Uwatoko, S., Kinoshita, M., Sumiya, M.: "Up-regulation of intercellular adhesion molecule-1(ICAM-1), endothelial leukocyte adhesion molecule-1(ELAM-1)and class II MHC molecules on pulmonary artery endothelia

Okawa-Takatsuji, M., Aotsuka, S., Fujinami, M., Uwatoko, S., Kinoshita, M., Sumiya, M.：“细胞间粘附分子-1 (ICAM-1)、内皮白细胞的上调