CHARACTERIZATION OF RAT THERMOSTATS

We have identified that warm sensitive cells in the hypothalamus and warm-receptors in the skin act as thermostats for thermoregulation.Patch clamp studies of hypothalamic warm-sensitive neurons suggest that warming-activated cation channel is key factor of the thermostats.The purpose of this study is molecular cloning of genes relevant to the thermostat.We used F-11(Cell Line Derived From Dorsal Root Ganglion)as a source of mRNA.1。Warming increased intracellular Ca I D 12 I 1 level[Ca I D 12 I D 1]I in F-11 cells，detected by Ca Fura-2 AM.2.Warming induced whole-cell inward current in F-11 cells voltage clamped at-60 mV.3.Oocytes infected with F-11mRNA responded to warming，detected by two-electrode voltage-clamp method。First，warming induced current continuously Second，warming induced current transiently and inactivated soon.We plan to make f-11cDNA library for molecular cloning of genes relevant to thermostats.

We have identified that warm sensitive cells in the hypothalamus and warm-receptors in the skin act as thermostats for thermoregulation. Patch clamp studies of hypothalamic warm-sensitive neurons suggest that warming-activated cation channel is key factor of the thermostats. The purpose of this study is molecular cloning of genes relevant to the thermostat. We used F-11 (cell line derived from dorsal root ganglion) as a source of mRNA.1. Warming increased intracellular CaィイD12+ィエD1 level [CaィイD12+ィエD1]I in F-11 cells, detected by Ca Fura-2AM.2. Warming induced whole-cell inward current in F-11 cells voltage clamped at -60mV.3. Oocytes infected with F-11 mRNA responded to warming, detected by two-electrode voltage-clamp method. First, warming induced current continuously Second, warming induced current transiently and inactivated soon.We plan to make f-11 cDNA library for molecular cloning of genes relevant to thermostats.