CHARACTERIZATION OF RAT THERMOSTATS

大鼠恒温器的特性

• 批准号: 09670069
• 负责人: KOBAYASHI Shigeo
• 金额: $ 2.3万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670069/
• 关键词: THERMOSTAT; CLONING; WARM; CHANNEL; mRNA; 発現クローニング; cDNAライブラリー; 後根神経節; 視床下部; 卵母細胞; 冷受容ニューロン; 温度受容ニューロン; F-11

We have identified that warm sensitive cells in the hypothalamus and warm-receptors in the skin act as thermostats for thermoregulation. Patch clamp studies of hypothalamic warm-sensitive neurons suggest that warming-activated cation channel is key factor of the thermostats. The purpose of this study is molecular cloning of genes relevant to the thermostat. We used F-11 (cell line derived from dorsal root ganglion) as a source of mRNA.1. Warming increased intracellular CaィイD12+ィエD1 level [CaィイD12+ィエD1]I in F-11 cells, detected by Ca Fura-2AM.2. Warming induced whole-cell inward current in F-11 cells voltage clamped at -60mV.3. Oocytes infected with F-11 mRNA responded to warming, detected by two-electrode voltage-clamp method. First, warming induced current continuously Second, warming induced current transiently and inactivated soon.We plan to make f-11 cDNA library for molecular cloning of genes relevant to thermostats.

我们已经发现，下丘脑中的热敏感细胞和皮肤中的热感受器充当体温调节的恒温器。对下丘脑温敏神经元的膜片钳研究表明，温敏激活的阳离子通道是温控的关键因素。本研究的目的是克隆与恒温器相关的基因。我们使用F-11(来源于背根神经节的细胞系)作为mRNA1的来源。结果：1.温热使F-11细胞内[CaィイD12+ィエD1]i水平升高，[CaィイD12+ィエD1]i升高。加温诱导F-11细胞全细胞内向电流，钳制电压为-60 mV.3。用双电极电压钳方法检测感染F-11mRNA的卵母细胞对温育的反应。首先是持续升温诱导电流，其次是瞬时升温诱导电流，然后是失活电流。我们计划建立f-11基因文库，用于恒温器相关基因的分子克隆。

项目成果

KOBAYASHI, Shigeo: "Seitai Joho Shori"Korona-sha. 200 (2000)

小林茂夫：《Seitai Joho Shori》Korona-sha。

小林茂夫: "生体情報処理"コロナ社. 200 (2000)

小林茂雄：《生物信息处理》Coronasha 200 (2000)。

Shigeo Kobayashi: "Warming activated channels of warm-sensitive neurons in rat hypothalamic slices"Neuroscience Letters. 273. 1-4 (1999)

Shigeo Kobayashi：“大鼠下丘脑切片中热敏感神经元的变暖激活通道”神经科学快报。

小林茂夫: "生体情報処理-生体サーモスタットから見えてくる生体情報系" コロナ社, 230 (2000)

Shigeo Kobayashi：“生物信息处理 - 从生物恒温器中看到的生物信息系统”Coronasha，230（2000）