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Studies on the regulator of food intake in the neonatal chick

新生雏鸡摄食调节的研究

基本信息

批准号：
09660319
负责人：
FURUSE Mitsuhiro
金额：
$ 2.11万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660319/
关键词：
GLP-1 CRF NPY CNS Food intake Chick Fasting Exendin ニワトリヒナ摂食行動コルチコステロン中枢絶食

项目摘要

RecentIy, it was reported that centraI abministrati of GLP-l decreased food intake in rats. The amino acid sequences of GLP-1 are identical in all mammals, and chicken GLP-1 exhibits a high homology with mammalian GLP-1. The aim of first experiment was to elucidate whether central injection of mammalian or chicken GLP-l inhibits food intake in the chick, and to compare their effects. IntracerebroventricuIar administration of mammalian and chicken GLP-1 strongly inhibited food intake of chicks. However, the suppressive effect of both GLP-1 on food intake was similar. These results show that GLP-1 with a variety of amino acid sequences may be the most potent inhibitor of food intake in the chicken. The aim of second experiment was to elucidate whether suppressed food intake by central injection of GLP-1 would be modified by an appetite stimulant such as fasting and neuropeptide Y (MN). Birds starved for 6 h ate significantly more food than those starved for 3 h, while irrespective of the … More time for fasting GLP-1 strongly inhibited food intake as rapidly as 10 min after i.c.v injection., Central administration of NPY greatly enhanced food intake, but co-injection of GIP-1 (0.1,02 or 0.3 IOTAg/1O IOTA1) decreased food Intake in a dose-dependent fashion. The aim of third experiment was to elucidate whether the suppression of food intake induced by central injection of GLP-1 is mediated by activation of the hypothalamic-pituitary-adrenal axis. Fist, the effects of central administration of corticotropin-reIeasing factor (CRF) were investigated CRF strongly inhibited food intake. Thereafter, effects of central CRF or GLP-1 on plasma corticosterone concentration were examined CRF significantly stimulated corticosterone release, but GLP- I did not alter plasma corticosterone concentration These results suggest that CRF is a potent inhibitor of food intake in the chick, but the suppression of food intake induced by central GLP-1 may not involve the activation of hypothaIamic -pituitary-adrenal axis. Furthermore, it was dear that C-terminal amino acids of GLP-1(7-36) have an important role for the bioactivity in the central nervous system with special reference to feeding behavior in the fourth experiment and that modification of a part of ammo acid sequence of exendin (9-39) may be required to antagonize chicken GLP-1 receptor in fifth experiment Less

最近，据报道，GLP-1的中枢给药减少了大鼠的食物摄入。GLP-1的氨基酸序列在所有哺乳动物中是相同的，并且鸡GLP-1与哺乳动物GLP-1表现出高度同源性。第一个实验的目的是阐明中枢注射哺乳动物或鸡GLP-1是否抑制鸡的食物摄取，并比较它们的效果。侧脑室注射哺乳动物和鸡GLP-1强烈抑制鸡的摄食。然而，两种GLP-1对摄食的抑制作用相似。这些结果表明，具有多种氨基酸序列的GLP-1可能是鸡中最有效的摄食抑制剂。第二个实验的目的是阐明通过中枢注射GLP-1抑制的食物摄入是否会被食欲刺激剂如禁食和神经肽Y（MN）改变。饥饿6 h的鸡比饥饿3 h的鸡进食量显著增加， ...更多信息空腹时间GLP-1强烈抑制食物摄入，快至i.c.v注射后10分钟，中枢给予NPY可显著增加摄食量，而联合注射GIP-1（0.1，02或0.3IOTAg/10 IOTA 1）则呈剂量依赖性降低摄食量。第三个实验的目的是阐明通过中枢注射GLP-1诱导的摄食抑制是否由下丘脑-垂体-肾上腺轴的激活介导。首先，研究了促肾上腺皮质激素释放因子（CRF）中枢给药的作用。结果表明，CRF能显著促进皮质酮的释放，而GLP-1对血浆皮质酮浓度无明显影响。这些结果表明，CRF是一种有效的摄食抑制剂，但中枢GLP-1对摄食的抑制可能不涉及下丘脑-垂体-肾上腺轴的激活。此外，在第四个实验中清楚的是GLP-1（7-36）的C-末端氨基酸对于中枢神经系统中的生物活性具有重要作用，特别是关于摄食行为，并且在第五个实验中可能需要对exendin（9-39）的部分氨基酸序列进行修饰以拮抗鸡GLP-1受体。