Analysis of gene conversions between tandem duplicated genes

串联重复基因之间的基因转换分析

• 批准号: 09640741
• 负责人: SAITOU Naruya
• 金额: $ 1.92万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640741/
• 关键词: molecular evolution; moplecular phylogenetic tree; phylogenetic network; Rh blood group; ABO血液型; 最大法

We have sequenced the complete coding region of the Rh blood group gene for mouse and rat and that of Rh-related 50kD glycoprotein (Rh5O) for mouse, rat, and crab-eating macaque. Phylogenetic analyses of Rh and Rh5O amino acid sequences indicate that the Rh5O gene has been evolving about two times more slowly than the Rh blood group gene both in primates and rodents. This conservative nature of the Rh5O gene suggests its relative importance to the Rh blood group gene. The time of gene duplication that produced the Rh and Rh50 genes was estimated to be about 240-310 million years ago. We also conducted window analyses of synonymous and nonsynonymous nucleotide substitutions for those two genes. Some peaks where nonsynonymous substitutions are higher than synonymous ones were located on outer membrane regions. This suggests the existence of positive Darwinian selection on Rh and Rh5O genes through host-parasite interactions.

我们测定了小鼠和大鼠Rh血型基因的全编码区和小鼠、大鼠和食蟹猕猴Rh相关的50 kD糖蛋白（Rh 5 O）的全编码区。Rh和Rh 5 O氨基酸序列的系统发育分析表明，在灵长类和啮齿类动物中，Rh 5 O基因的进化速度比Rh血型基因慢两倍。Rh 5 O基因的这种保守性表明其相对于Rh血型基因的重要性。据估计，产生Rh和Rh 50基因的基因复制时间约为2.4亿至3.1亿年前。我们还对这两个基因的同义和非同义核苷酸替换进行了窗口分析。一些峰，其中非同义取代高于同义的位于外膜区域。这表明存在积极的达尔文选择Rh和Rh 5 O基因通过宿主-寄生虫的相互作用。

项目成果

Saitou N.and Yamamoto F: "Evolution of primate ABO blood group genes and their homologous genes" Molecular Biology and Evolution. Vol.14, No.4. 399-411 (1997)

Saitou N.和 Yamamoto F：“灵长类 ABO 血型基因及其同源基因的进化”《分子生物学与进化》。

Saitou N.: "Evolution of primate ABO blood group genes and their homologous genes" Molecular Biology and Evolution. 14・4. 399-411 (1997)

Saiton N.：“灵长类ABO血型基因及其同源基因的进化”《分子生物学与进化》14・4（1997）。

斎藤成也: "遺伝子は35億年の夢を見る-バクテリアから人間の進化まで-" 大和書房, 東京, 229 (1997)

齐藤圣哉：《35亿年的基因梦想——从细菌到人类进化》大和书房，东京，229（1997）

斎藤成也: "遺伝子の進化とは〜ABO式血液型を中心に〜" BIO Clinica. 12・11. 830-834 (1997)

Seiya Saito：“什么是基因进化？关注 ABO 血型”BIO Clinica 12・11（1997）。

Kitano T., Sumiyama T., Shiroishi T., and Saitou N.: "Conserved evolution of the Rh50 gene compared to its homologous Rh blood group gene" Biochemical and Biophysical Research ommunications. vol. 249, No. 1. 78-85 (1998)

Kitano T.、Sumiyama T.、Shiroishi T. 和 Saitou N.：“Rh50 基因与其同源 Rh 血型基因相比的保守进化”生物化学和生物物理研究通讯。