Analysis of between-species and intra-species diversity using primate genome data and application to conservation biology

为了研究灵长类动物的保护生物学和种内多样性，我们开发了Prim-Prim数据库。通过对人类和猕猴基因组序列的比较，提取出两种物种间具有相同外显子序列夹心的内含子区域，将2303对可扩增灵长类动物基因组未测序DNA片段的引物纳入该数据库，并对外开放。我们用这些引物对6个长臂猿物种的核苷酸序列进行了测定，结果表明引物的有效性。

For primate conservation biology and for intraspecies diversity study, we developed database called "Prim-Prim". Through comparison of human and macaque genomic sequences, intronic regions sandwitched with identical exonic sequences between two species were extracted, and 2,303 primer pairs which can amplify DNA fragment of unsequenced primate genomes were listed in this database, and it is open to public. We used these primers to determine nucleotide sequences of 6 gibbon species, and showed usefulness of these primers.