Primate Comparative Genome Analysis and Study on Mechanisms of Human Evolution

灵长类比较基因组分析与人类进化机制研究

• 批准号: 14011248
• 负责人: SAITOU Naruya
• 金额: $ 76.8万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14011248/
• 关键词: human evolution; molecular evolution; ehromnsorne; ape; genome evolution

Our most prominent achievement was determination and evolutionary analysis of chimpanzee chromosome 22 long arm genomic sequences, published in Nature in 2004. Saitou and Fujiyama were both corresponding authors of this paper. Detailed difference on amino acid sequences and genomic sequences were delineated. In particular, clear difference between insertion and deletion was discovered. We also used chimpanzee genome sequence as outgroup to infer direction of mutations that created human SNP, and estimated nucleotide substitution patterns. Another large scale study was determination of BAC-end sequences of chimpanzee BAC library, and human-chimpanzee difference in terms of nucleotide substitution was determined to be 1.23% (Fujiyama et al., Science, 2002). Gorilla fosmid library was also constructed (Kim et al., Genomics,2003).We also compared 103 protein coding gene sequences for human, chimpanzee, gorilla, and orangutan (Kitano et al., Molecular Biology and Evolution, 2004). Various statistical tests were used to detect human specific changes, but each test picked up different set of genes. Other results include various types of human and ape comparison on SCA triplet repeat polymorphism (Andres et al, Journal of Molecular Evolution) and polyubiquitin (Tachikui et al., Journal of Molecular Evolution), as well as mitochondrial DNA polymorphism study on African wild chimpanzees (Shimada et al., American Journal of Primatology).

我们最突出的成就是对黑猩猩22号染色体长臂基因组序列的测定和进化分析，发表在2004年的《自然》杂志上。Saitou和Fujiyama都是这篇论文的通讯作者。详细描述了氨基酸序列和基因组序列的差异。特别是，插入和缺失之间的明显差异被发现。我们还使用黑猩猩基因组序列作为外群来推断产生人类SNP的突变方向，并估计核苷酸取代模式。另一个大规模研究是确定黑猩猩BAC文库的BAC末端序列，并且人-黑猩猩在核苷酸取代方面的差异被确定为1.23%（Fujiyama等人，Science，2002）。还构建了大猩猩fosmid文库（Kim等人，我们还比较了人类、黑猩猩、大猩猩和猩猩的103种蛋白质编码基因序列（Kitano等人，分子生物学和进化，2004）。各种统计测试被用来检测人类特定的变化，但每一个测试都选择了不同的基因组。其他结果包括在SCA三联体重复多态性（Andres等人，Journal of Molecular Evolution）和多聚泛素（Tachikui等人，Journal of Molecular Evolution），以及对非洲野生黑猩猩的线粒体DNA多态性研究（Shimada et al.，美国灵长类动物学杂志（American Journal of Primatology）

项目成果

ゲノウと進化-ゲノムから立ち昇る生命-

Genou 和进化 - 生命从基因组中崛起 -

• 发表时间: 2004
• 作者: Komano J;Miyauchi K;Matsuda Z;Yamamoto N.;Watanabe Y. et al.;斎藤 成也
• 通讯作者: 斎藤 成也

Point Matching Under Non-Uniform Distortions and Protein Side Chain Packing Based on an Efficient Maximum Clique Algorithm.

基于高效最大团算法的非均匀畸变和蛋白质侧链堆积下的点匹配。

• 发表时间: 2002
• 期刊: Genome Informatics 13
• 作者: Yamamoto;M.;Yamazaki;S.;Uematsu;S.;Sato;S.;Hemmi;H.;Hoshino;K.;Kaisho;T.;Kuwata;H.;Takeuchi;O.;Saitoh;T.;Yamaoka;S.;Yamamoto;N.;Yamamoto;S.;Muta;T.;Takeda;K.;Akira;S.;Bahadur D.
• 通讯作者: Bahadur D.

Identification of two novel primate-specific genes in DSCR

• DOI: 10.1093/dnares/9.3.89
• 发表时间: 2002-06-30
• 期刊: DNA RESEARCH
• 影响因子: 4.1
• 作者: Takamatsu, K;Maekawa, K;Takeda, T
• 通讯作者: Takeda, T

Construction of a bacterial artificial chromosome library from the inshore hagfish, Eptatretus burgeri : A resourece for the analysis of the agnathan genome.

近海盲鳗 Eptatretus Burgeri 细菌人工染色体文库的构建：无颌基因组分析的资源。

• 发表时间: 2004
• 期刊: Genes ＆ Genetic Systems 79
• 作者: Mori K;Sugimoto C;Ohgimoto S;Nakayama EE;Shioda T Kusagawa S;Takebe Y;Kano M;Matano T;Yuasa T;Kitaguchi D Miyazawa M;Takahashi Y;Yasunami M;Kimura A;Yamamoto N Suzuki Y;Nagai Y.;Tachikui H.;Suzuki T.
• 通讯作者: Suzuki T.

Duan J.: "Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor"Human Molecular Genetics. 12・3. 205-216 (2003)

Duan J.：“人类多巴胺受体 D2 (DRD2) 的同义突变影响受体的 mRNA 稳定性和合成”人类分子遗传学 12・3 (2003)。