Study of the development a new type of treatment of urogenital cancer by using surface modified fine metal particles.

利用表面改性细金属颗粒开发新型治疗泌尿生殖系统癌症的研究。

• 批准号: 10557143
• 负责人: KUBOTA Yoshinobu
• 金额: $ 3.97万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557143/
• 关键词: Fine metal particles; TiO_2; Photocatalysis; Urogenital Cancer; Cancer treatment; 酸化チタン; 遺伝子治療

We tried to develop a new type of treatment of cancer by using specifically surface modified fine metal particles, especially particles coated by TiO_2 photacatalyst. They might be also applicable to gene therapy without using virus vector.1) TiO_2 coated fine gold particles were successfully developed and they were shown to be very stable and safe in tissues or cells. The TiO_2 coated gold particles showed photacatalytic activities when there were exposed to 360 nm UV light. The photacatalytic capacity of them were dependent on the amount of TiO_2 coated on the gold particles.2) The growth of human prostate cancer PC-3 tumors which were grown on nude mice, and lost P53 genes expression, were suppressed dramatically by the enforced introduction of wild type P53 gene using non- viral gene transfer systems.3) The growth of human bladder cancer T-24 tumors on nude mice were effectively suppressed by the combined treatment of 360 nm UV light exposure and TiO_2 coated gold particles which was introduced into the cancer tissues by physiological enforced method. These results suggest that the combination of TiO_2 coated fine gold particle and UV light could be one of the new types of cancer treatment.

我们试图通过使用特定表面改性的金属细颗粒，特别是涂有TiO_2光催化剂的颗粒来开发一种新型的癌症治疗方法。1）成功研制了纳米TiO_2包裹的金微粒，其在组织或细胞中的稳定性和安全性都很好。在360 nm紫外光照射下，TiO_2包覆的金颗粒表现出光催化活性。2）在裸鼠体内培养的人前列腺癌PC-3细胞瘤的生长情况，P53基因表达缺失，通过非病毒基因转移系统导入野生型P53基因，可显著抑制人膀胱癌T细胞的生长。用360 nm紫外光照射和生理性强化法将TiO_2包裹金粒子导入裸鼠肿瘤组织，对24个裸鼠肿瘤进行了有效的抑制。这些结果表明，TiO_2包覆金微粒与紫外光的结合可能是一种新型的癌症治疗方法。

项目成果

Noguchi S et al.: "High alkaline phosphatase activity in 3p-induced human renal cancer cells"Cancer Letters. 131. 223-227 (1998)

Noguchi S 等人：“3p 诱导的人肾癌细胞中碱性磷酸酶活性高”Cancer Letters。

Noguchi S. Shuin T. Kubota Y. Watanabe S. Nagashima Y. Masuda M. Yao M, Hosaka M.: "High alkaline phosphatase activity in 3p-induced human renal cancer cells"Cancer Letters. 131. 223-227 (1998)

Noguchi S. Shuin T. Kubota Y. Watanabe S. Nagashima Y. Masuda M. Yao M, Hosaka M.：“3p 诱导的人肾癌细胞中碱性磷酸酶活性较高”《癌症快报》。

Popa E. <Kubota Y.> Tryk D.A. Fujishima A.: "Selective Voltarametric and Araperometric Detection of Uric acid with Oxidized Diamond Film Electrodes"Analytical Chemistry. 72. 1724-1727 (2000)

Popa E. <久保田 Y.> Tryk D.A.

Ohko Y. Tatuma T. Utumi Y. Kobayakawa K. Satoh Y. Niwa C. Kubota Y. Fujishima A: "Self-sterlizing and self-cleaning of silicone catheters coa|ted with Ti0_2 phtocatalyst thin films"J. Biomedical. Materialas Research. 58. 97-101 (2001)

Ohko Y. Tatuma T. Utumi Y. Kobayakawa K. Satoh Y. Niwa C. Kubota Y. Fujishima A：“涂有Ti0_2光催化剂薄膜的硅胶导管的自消毒和自清洁”J。

Ohko Y. Ando I. Niwa C. Tatsuma T, Yamamura T. Nakashima T. Kubota Y. Fujishima A.: "Degradation of bisphenol-A in water by Ti02 photcatalysis"Environmental Science and Technology. 35. 2365-2368 (2001)

Ohko Y. Ando I. Niwa C. Tatsuma T、Yamamura T. Nakashima T. Kubota Y. Fujishima A.：“TiO2 光催化降解水中双酚 A”环境科学与技术。