Providing titanium for Bone Substitution with Bioactivity by treatment with Hydrogenperoxide containing Metal Chlorides

在种植体上形成磷灰石对于实现与骨组织的直接结合是很重要的。我们最近表明，用含有氯化钽的过氧化氢溶液进行化学处理的金属钛能够在具有类似于人类血浆的无机离子组成的模拟体液中形成羟基磷灰石层。在本研究中，为了研究该化学治疗在临床应用中的潜力，我们将经该方法处理的纯钛圆柱体植入兔胫骨的一个孔中，使其具有骨结合能力。植入16周后，将标本与骨组织一起取出，通过推出试验评估植入物与骨组织之间的剪切力。结果与未处理的纯钛进行了比较。术后8周，治疗组植入4.2mm孔的钛材剪切力明显高于未治疗组，但表面粗糙度未发生变化。扫描电镜（SEM）和能量色散x射线（EDX）显微分析表明，骨非常接近处理过的钛的表面。而且，植入样品在4.0mm孔中的剪切力比在4.2mm孔中的剪切力要大。由此，证实了含氯化钽的双氧水溶液处理对体内钛种植体有较高的结合能力。

Apatite formation on implant is important in achieving a direct bonding to bone tissue. We recently showed that titanium metal chemically treated with a hydrogen peroxide solution containing tantalum chlorides has ability to form a hydroxyapatite layer in simulated body fluid which had inorganic ion composition similar to human blood plasma. In this study, to investigate potential of this chemical treatment for clinical application, bone-bonding ability of a pure titanium cylinder treated with this method was implanted into a hole in a rabbit's tibia. After implantation for predetermined periods up to 16 weeks, the specimens were extracted with bone tissue, and were examined by push-out test to evaluate the shearing force between the implant and bone tissue. The results were compared with those of non-treated pure titanium. Eight weeks after surgery, the shearing force of the treated titanium implanted in 4.2mm-hole was significantly higher than that of non-treated titanium, although he surface roughness was not changed after the treatment. Scanning electron microscopic (SEM) observation and energy dispersive-X-ray (EDX) microanalysis showed that the bone comes very close to surface of treated titanium. Moreover, the shearing force was higher for the implanted sample in the 4.0mm-hole, than that in 4.2mm-hole. Thus, it is confirmed that treatment with hydrogen peroxide solution containing tantalum chlorides higher bonding ability on titanium implants in vivo.