Providing titanium for Bone Substitution with Bioactivity by treatment with Hydrogenperoxide containing Metal Chlorides

种植体表面磷灰石的形成对实现与骨组织的直接结合具有重要意义。我们最近发现，用含有氯化钽的过氧化氢溶液化学处理的金属钛在模拟体液中能够形成一层羟基磷灰石，其无机离子组成类似于人类血浆。为了探讨这种化学处理方法的临床应用潜力，将用这种方法处理的纯钛圆柱体的骨结合能力植入兔胫骨的一个洞中。种植16周后取出标本，用推出法测定种植体与骨组织间的剪切力。并与未处理纯钛的结果进行了比较。术后8周，表面粗糙度无明显变化，但经处理的钛植入4.2 mm孔的剪切力明显高于未处理的钛。扫描电子显微镜(SEM)观察和能量色散X射线(EDX)微观分析表明，处理后的钛骨与表面非常接近。此外，4.0 mm孔中植入的样品的剪切力大于4.2 mm孔中植入的样品。由此可见，含氯化钽的过氧化氢溶液在体内对钛种植体具有较高的粘结能力。

Apatite formation on implant is important in achieving a direct bonding to bone tissue. We recently showed that titanium metal chemically treated with a hydrogen peroxide solution containing tantalum chlorides has ability to form a hydroxyapatite layer in simulated body fluid which had inorganic ion composition similar to human blood plasma. In this study, to investigate potential of this chemical treatment for clinical application, bone-bonding ability of a pure titanium cylinder treated with this method was implanted into a hole in a rabbit's tibia. After implantation for predetermined periods up to 16 weeks, the specimens were extracted with bone tissue, and were examined by push-out test to evaluate the shearing force between the implant and bone tissue. The results were compared with those of non-treated pure titanium. Eight weeks after surgery, the shearing force of the treated titanium implanted in 4.2mm-hole was significantly higher than that of non-treated titanium, although he surface roughness was not changed after the treatment. Scanning electron microscopic (SEM) observation and energy dispersive-X-ray (EDX) microanalysis showed that the bone comes very close to surface of treated titanium. Moreover, the shearing force was higher for the implanted sample in the 4.0mm-hole, than that in 4.2mm-hole. Thus, it is confirmed that treatment with hydrogen peroxide solution containing tantalum chlorides higher bonding ability on titanium implants in vivo.