Preparation of self-organized nano-structured apatite and the protein adsorption property

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The selective protein adsorption property and the local structure around carbonate ions of nanocrystalline hydroxy-carbonate apatite were examined. Considerable changes in the selectivity in the adsorption of BSA and β_2-MG were observed due to the incorporation of the carbonate ions in hydroxyapatite lattice. The chemical states of the incorporated carbonate ions were examined by the 2D NMR spectroscopy. At least four or five peaks assignable to carbonate ions in A-site(OH) and B-site(PO_4^3) were observed in 2D ^<13>C{^1H} or ^<31>P{^1H} Het-Cor NMR spectroscopy. The surface charge distribution and the decrement of polar groups such as OH groups due to the distribution of carbonate ions in both A-and B-sites of the hydroxyapatite lattice are particularly favorable for β_2-MG adsorption rather than for BSA adsorptionWe prepared nano-crystalline Zn-containing hydroxyapatite (ZnHAp) by the wet-chemical method and examined the selective adsorption of essential proteins, taking bovine serum albumin (BSA) and pathogenic protein such as β_2-microglobulin (β_2-MG) as model proteins. The increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing the Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing the Zn content in spite of the increase in the specific surface area. As the Zn^<2+> ion content in the apatites increased, the adsorbed amount of BSA was almost constant, whereas that of β_2-MG increased