Mechanical property and bioactivity of organic-inorganic hybrid materials containing silanol groups

We synthesized calcium-containing organically modified silicates （Ormosil） starting from ploy （dimethylsiloxane） （PDMS， -（SiO（CH_D23_D2）_D2n_ One of the typical compositions for starting materials was TEOS ： PDMS ： HCl ： HイイD22エD2O ： Ca（NOイD23エD2）イD22エD2 ·4 HイD22エD2O = 1 ： 1.64 ： 0.1 ： 3 ： 0.1 （molar ratio）。We investigated effs of HCl and Ca（NOイイD23イD2）イD22イD2 ·4イD22イD2O contents in gelling solutions on bioactivity and microstructure of Ormoosils-type samples。I-D129 I-D1 Si MAS核磁共振特别指标表明，HCl的数量没有显示对硅原子周围地方结构的任何显著影响。对Ormosils-type样本的碎片表面的SEM-EDX分析显示了与其结构相关的Ca的孔大小和数量。我们把更大的生物活性结合到了Incorpo上 ... More ration of more calcium ions in the polymer due to addition of larger amounts of HCl and /or Ca（NOイイD23イエD2）イイD22イイD2 ·4イイD2O。Ormos sols prepared from PDMS-TEOS-Ca with /without addition of DMF were coated on Nylon 6 [R! * * 和硅基材料，配有浸渍涂层技术。涂层层是1.5微米的厚度，没有裂缝。涂层薄膜和基材之间的粘合强度不小于约1.7兆帕。没有DMF的电影没有在SBF的14天内存放一块，而在SBF的7天内存放在DMF的14天内。薄膜DMF和无DMF薄膜在微结构之间的差异解释了生物活性中的差异。自AEROSIL [R!]是硅的强化材料之一，我们合成了用AEROSIL强化的ORMOSIL凝胶（R!（）starting from PDMS， TEOS and Ca（NOイイD23イエD2）イイD22イエD2 ·4イイD22イエD2O through sol-gel processing。用航空器增加的储存模块（R!与PDMS-AEROSIL中的增加有关的内容（R!（b）债券。gel （molar ratio， TEOS ： PDMS ：H-D22-D2O ： HCl ： CA（NO-D23-D2）-D22-D2 = 1 ： 0.24 ： 5.0 ： 0.9 ： 0.25） containing 5 g AEROSIL [R!（F5H0.6 Ca0.25） could deposit apatite within 1 day of soaking in the Kokudo solution， since F5H0.6 Ca0.25 included many calcium ions on the surface.Cytocompatibility of organic-inorganic hybrids reinforced with AEROSIL [R!是的，被核实了。类似于骨芽的细胞（MC 3 T3-E1）在36.5 ° C以下5%和95%的人类特征上被培养出来。Cell numbers with specimen after incubation for 3 and 7 Days showed higher degree of proliferation than that without specimen denoted as "blank"。怎么办，这是不值得赞赏的，因为在细胞测试中，细胞数的空白在孵化后的3到7天内被减少了1/10。无论如何，这个结果都是根据混合体没有显示细胞毒性的。Less（低）

We synthesized calcium-containing organically modified silicates (Ormosils) starting from ploy (dimethylsiloxane) (PDMS, -(SiO(CHィイD23ィエD2)ィイD22ィエD2)ィイD2nィエD2-), tetraethoxysilane (TEOS), Si(OCィイD22ィエD2HィイD25ィエD2)ィイD24ィエD2) and calcium nitrate (Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O). One of the typical compositions for starting materials was TEOS : PDMS : HCl : HィイD22ィエD2O : Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O = 1 : 1.64 : 0.1 : 3 : 0.1 (molar ratio). We investigated effects of HCl and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O contents in gelling solutions on bioactivity and microstructure of Ormosils-type samples. Analysis of ィイD129ィエD1Si MAS NMR spectra indicated that amounts of HCl do not show any significant effects for local structure around Si atoms. The SEM-EDX analysis of fracture-surface for Ormosils-type sample showed that the pore size and amount of Ca incorporated in their structure depended on HCl amounts. We concluded that greater bioactivity was attributed to incorpo … More ration of more calcium ions in the polymer due to addition of larger amounts of HCl and /or Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O.The Ormosils sols prepared from PDMS-TEOS-Ca with /without addition of DMF were coated on Nylon6【R!〇】 and silicone substrates with a dip-coating technique. The coated layer was 1.5μm thickness without cracks. Adhesive strength between the coated films and substrates was not less than about 1.7 MPa. The DMF-free film did not deposit apatite within 14 days while the films from the sols with DMF deposited apatite within 7 days in SBF. The difference in microstructure between film-DMF and DMF-free film explained that in bioactivity.Since AEROSIL【R!〇】 is one of the reinforcement materials for silicone, we synthesized the ORMOSILS gels reinforced with AEROSIL【R!〇】 starting from PDMS, TEOS and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O through sol-gel processing. The storage modulus increased with the AEROSIL【R!〇】 content along with the increase in PDMS-AEROSIL【R!〇】 bonds. The gel (molar ratio, TEOS : PDMS : HィイD22ィエD2O : HCl : CA(NOィイD23ィエD2)ィイD22ィエD2 = 1 : 0.24 : 5.0 : 0.9 : 0.25) containing 5g AEROSIL【R!〇】 (F5H0.6Ca0.25) could deposit apatite within 1 day of soaking in the Kokudo solution, since F5H0.6Ca0.25 included many calcium ions on the surface.Cytocompatibility of organic-inorganic hybrids reinforced with AEROSIL【R!〇】 was examined. Osteoblast-like cells (MC3T3-E1) derived from mouse were cultured on the specimens at 36.5℃ under 5% and 95% humidity. Cell numbers with specimen after incubation for 3 and 7days showed higher degree of proliferation than that without specimen denoted as "blank". However, it was not appreciable because the cell numbers of blank after incubation for 3 and 7days reduced by 1/10 in comparison with usual case in cell examination. Anyway, this results indicated that the hybrids did not show cytotoxicity. Less