Mechanical property and bioactivity of organic-inorganic hybrid materials containing silanol groups

We synthesized calcium-containing organically modified silicates （Ormosils）从ploy开始（dimethylsiloxane） （PDMS, -(SiO(CH - D23 - D2) - D22 - D2) - D2n - D2-）， tetraethoxysilane (TEOS)，Si(OC D22 D2H D25 D25 D2) and calcium nitrate (Ca(NO D23 D23 D2) D22 D22 D22 D2O).One of the typical compositions for starting materials was TEOS: PDMS: HCl: H - 22 - D2O：Ca(NO - D23 - D2) D22 - D2 - 4H - D22 - D2O = 1: 1.64: 0.1: 3：0.1 (molar ratio). We investigated effects of HCl and Ca(NO - D23 - D2) D22 - D2 - 4h - D22 - D2Ocontents in gelling solutions on bioactivity and microstructure of Ormosils-type samples. AnalysisD1Si MAS NMR spectra indicated that amounts of HCl不显示任何有意义的效果for local structure around Si atoms. The SEM-EDX analysis of fracture-surface for Ormosils-typesample showed that the pore size and amount of Ca incorporated in their structure depended on HClamounts.我们包含的greater bioactivity was attributed to incorpo…更多calcium ions in the polymer due to addition of larger amounts of HCl and/or Ca(NO - D23 - D2) D22 - D2 - 4h D22 - D2O.The Ormosils sols prepared from PDMS-TEOS-Ca with/without addition of DMF were coated on Nylon6and silicone substrates with a daip -coating技术nique. The coated layer was 1.5μm thicknesswithout cracks. Adhesive strength between the coated films and substrates was not than about1.7 MPa. The DMF-free film did not deposit apatite within 14 days while The films from The sols withDMF deposited apatite within 7 days in SBF. The difference in microstructure between film-DMF andDMF-free film explained that in bioactivity.Since AEROSIL【R！is one of the reinforcement materials for siliconewe synthesized the ORMOSILS gels reinforced with AEROSIL从PDMS开始，TEOS and Ca(NO - D23 - D2) D22 - D2 - 4h - D22 - D2O through sol-gel processing. The storage modulusincreased with the AEROSIL【R!〇】content along with the increase in PDMS-AEROSIL【R！bonds. The gel (molar ratio, TEOS: PDMS: H - D22 - D2O: HCl: CA(NO - D23 - D2) D22 - D2 = 1：0.24: 5.0: 0.9：0.25) containing 5g AEROSIL【R！（f5h0.6 Ca0.25） could deposit apatite within 1 day of soaking in the Kokudo solution，since f5h 0.6克0.25包括many calcium ions on the surface.cytocompatibility of organic-inorganichybrids reinforced with AEROSIL【R！was examined. Osteoblast-like cells (MC3T3-E1) derived from mouse were cultured on the specimensat 36.5℃under 5% and 95% humidity. Cell numbers with specimen after incubation for 3 and 7days本应高学位的proliferation than that without specimen denoted as "blank". However，这并不适用，因为每个单元格的数字在blank after incubation for 3 and 7days删除1/10 in comparison with usual case in cell examination. Anyway，this results indicated that the hybrids did not show cytotoxicity. Less

We synthesized calcium-containing organically modified silicates (Ormosils) starting from ploy (dimethylsiloxane) (PDMS, -(SiO(CHィイD23ィエD2)ィイD22ィエD2)ィイD2nィエD2-), tetraethoxysilane (TEOS), Si(OCィイD22ィエD2HィイD25ィエD2)ィイD24ィエD2) and calcium nitrate (Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O). One of the typical compositions for starting materials was TEOS : PDMS : HCl : HィイD22ィエD2O : Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O = 1 : 1.64 : 0.1 : 3 : 0.1 (molar ratio). We investigated effects of HCl and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O contents in gelling solutions on bioactivity and microstructure of Ormosils-type samples. Analysis of ィイD129ィエD1Si MAS NMR spectra indicated that amounts of HCl do not show any significant effects for local structure around Si atoms. The SEM-EDX analysis of fracture-surface for Ormosils-type sample showed that the pore size and amount of Ca incorporated in their structure depended on HCl amounts. We concluded that greater bioactivity was attributed to incorpo … More ration of more calcium ions in the polymer due to addition of larger amounts of HCl and /or Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O.The Ormosils sols prepared from PDMS-TEOS-Ca with /without addition of DMF were coated on Nylon6【R!〇】 and silicone substrates with a dip-coating technique. The coated layer was 1.5μm thickness without cracks. Adhesive strength between the coated films and substrates was not less than about 1.7 MPa. The DMF-free film did not deposit apatite within 14 days while the films from the sols with DMF deposited apatite within 7 days in SBF. The difference in microstructure between film-DMF and DMF-free film explained that in bioactivity.Since AEROSIL【R!〇】 is one of the reinforcement materials for silicone, we synthesized the ORMOSILS gels reinforced with AEROSIL【R!〇】 starting from PDMS, TEOS and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O through sol-gel processing. The storage modulus increased with the AEROSIL【R!〇】 content along with the increase in PDMS-AEROSIL【R!〇】 bonds. The gel (molar ratio, TEOS : PDMS : HィイD22ィエD2O : HCl : CA(NOィイD23ィエD2)ィイD22ィエD2 = 1 : 0.24 : 5.0 : 0.9 : 0.25) containing 5g AEROSIL【R!〇】 (F5H0.6Ca0.25) could deposit apatite within 1 day of soaking in the Kokudo solution, since F5H0.6Ca0.25 included many calcium ions on the surface.Cytocompatibility of organic-inorganic hybrids reinforced with AEROSIL【R!〇】 was examined. Osteoblast-like cells (MC3T3-E1) derived from mouse were cultured on the specimens at 36.5℃ under 5% and 95% humidity. Cell numbers with specimen after incubation for 3 and 7days showed higher degree of proliferation than that without specimen denoted as "blank". However, it was not appreciable because the cell numbers of blank after incubation for 3 and 7days reduced by 1/10 in comparison with usual case in cell examination. Anyway, this results indicated that the hybrids did not show cytotoxicity. Less