Mechanical property and bioactivity of organic-inorganic hybrid materials containing silanol groups

含硅烷醇基有机无机杂化材料的力学性能和生物活性

• 批准号: 09450246
• 负责人: OSAKA Akiyoshi
• 金额: $ 7.1万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450246/
• 关键词: Organic-inorganic hybrid materials; Bioactive materials; Cytocompatibility; Mechanical property; Organically modified ceramics; Cell culture; Apatite; Silanol groups; 生体適合性; 機械的特性; 補強材; 有機・無機複合材料

We synthesized calcium-containing organically modified silicates (Ormosils) starting from ploy (dimethylsiloxane) (PDMS, -(SiO(CHィイD23ィエD2)ィイD22ィエD2)ィイD2nィエD2-), tetraethoxysilane (TEOS), Si(OCィイD22ィエD2HィイD25ィエD2)ィイD24ィエD2) and calcium nitrate (Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O). One of the typical compositions for starting materials was TEOS : PDMS : HCl : HィイD22ィエD2O : Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O = 1 : 1.64 : 0.1 : 3 : 0.1 (molar ratio). We investigated effects of HCl and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O contents in gelling solutions on bioactivity and microstructure of Ormosils-type samples. Analysis of ィイD129ィエD1Si MAS NMR spectra indicated that amounts of HCl do not show any significant effects for local structure around Si atoms. The SEM-EDX analysis of fracture-surface for Ormosils-type sample showed that the pore size and amount of Ca incorporated in their structure depended on HCl amounts. We concluded that greater bioactivity was attributed to incorpo … More ration of more calcium ions in the polymer due to addition of larger amounts of HCl and /or Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O.The Ormosils sols prepared from PDMS-TEOS-Ca with /without addition of DMF were coated on Nylon6【R!〇】 and silicone substrates with a dip-coating technique. The coated layer was 1.5μm thickness without cracks. Adhesive strength between the coated films and substrates was not less than about 1.7 MPa. The DMF-free film did not deposit apatite within 14 days while the films from the sols with DMF deposited apatite within 7 days in SBF. The difference in microstructure between film-DMF and DMF-free film explained that in bioactivity.Since AEROSIL【R!〇】 is one of the reinforcement materials for silicone, we synthesized the ORMOSILS gels reinforced with AEROSIL【R!〇】 starting from PDMS, TEOS and Ca(NOィイD23ィエD2)ィイD22ィエD2 ・ 4HィイD22ィエD2O through sol-gel processing. The storage modulus increased with the AEROSIL【R!〇】 content along with the increase in PDMS-AEROSIL【R!〇】 bonds. The gel (molar ratio, TEOS : PDMS : HィイD22ィエD2O : HCl : CA(NOィイD23ィエD2)ィイD22ィエD2 = 1 : 0.24 : 5.0 : 0.9 : 0.25) containing 5g AEROSIL【R!〇】 (F5H0.6Ca0.25) could deposit apatite within 1 day of soaking in the Kokudo solution, since F5H0.6Ca0.25 included many calcium ions on the surface.Cytocompatibility of organic-inorganic hybrids reinforced with AEROSIL【R!〇】 was examined. Osteoblast-like cells (MC3T3-E1) derived from mouse were cultured on the specimens at 36.5℃ under 5% and 95% humidity. Cell numbers with specimen after incubation for 3 and 7days showed higher degree of proliferation than that without specimen denoted as "blank". However, it was not appreciable because the cell numbers of blank after incubation for 3 and 7days reduced by 1/10 in comparison with usual case in cell examination. Anyway, this results indicated that the hybrids did not show cytotoxicity. Less

以聚二甲基硅氧烷（PDMS，-（SiO（CH ↓ [2] D ↓ [23] O ↓ [2] D ↓ [2]）O ↓ [22] O ↓ [2] D ↓ [2] n O ↓ [2]）、正硅酸乙酯（TEOS）、硅（OC ↓ [2] D ↓ [22] O ↓ [2] H ↓ [2] D ↓ [25] O ↓ [2] D ↓ [2]）和硝酸钙（Ca（NO ↓ [2] D ↓ [23] O ↓ [2] D ↓ [2]）O ↓ [2] D ↓ [22] O ↓ [2] D ↓ [2]·4 H ↓ [2] D ↓ [22] O ↓ [2]）为原料合成了含钙有机改性硅酸盐（Ormosils）。起始材料的典型组成之一是TEOS：PDMS：HCl：H2O D22·H2O D2 O：Ca（NO）2D 23·H2O D22·H2O D22·H2O D2 O = 1：1.64：0.1：3：0.1（摩尔比）。研究了凝胶溶液中HCl和Ca（NO）2 + D23-D2）2 + D22-D2 ·4 H-2 + D22-D2 O含量对Ormosils型样品生物活性和微观结构的影响。对イD129 D1 Si MAS NMR谱的分析表明，HCl的含量对Si原子周围的局部结构没有表现出任何显着影响。对Ormosils型样品的孔表面进行SEM-EDX分析表明，孔的大小和Ca的掺入量取决于HCl的量。我们的结论是，更大的生物活性归因于incorpo ...更多信息 通过添加大量的HCl和/或Ca（NO3-D23-D2）2-D22-D22-D2 O，使聚合物中的钙离子比例增加。Netherlands]和有机硅基材上进行的涂层。涂层厚度为1.5μm，无裂纹。涂膜和基材之间的粘合强度不小于约1.7MPa。在SBF中，无DMF的膜在14天内没有存款磷灰石，而来自含DMF的溶胶的膜在7天内沉积磷灰石。膜-DMF和无DMF膜之间微观结构的差异解释了生物活性。荷兰]是有机硅的增强材料之一，我们合成了AEROSIL[R！Netherland]起始于PDMS、TEOS和Ca（NO_（2-x）D_23）O_（2-x）D_22）O_（2-x）D_2·4 H_（2-x）D_22）O通过溶胶-凝胶处理。储能模量随着AEROSIL[R！沿着PDMS-AEROSIL[R！荷兰债券。将含有5g AEROSIL[R！Netherlands]（F5H0.6Ca0.25）在Kokudo溶液中浸泡1天后，由于F5H0.6Ca0.25表面含有大量的钙离子，可使磷灰石存款。荷兰）进行了审查。将小鼠成骨样细胞（MC 3 T3-E1）分别在36.5℃、5%和95%湿度下培养。培养3天和7天后，有标本的细胞数显示出比无标本的细胞（表示为“空白”）更高的增殖程度。然而，由于孵育3天和7天后的空白细胞数与细胞检查中的通常情况相比减少了1/10，因此并不明显。无论如何，该结果表明混合物未显示出细胞毒性。少

项目成果

Y. Aburatani他: "Synthesis of bioactive organic-inorganic hybrids reinforced with AEROSIL R"Bioceramics. 12. 457-460 (1999)

Y. Aburatani 等人：“用 AEROSIL R 增强的生物活性有机-无机杂化物的合成”生物陶瓷。 12. 457-460 (1999)

K. Tsuru, S. Hayakawa, C. Ohtsuki and A. Osaka: "Bioactivity of organically modified ceramics film Coated on Polymer Substrate"Bioceramics. Vol. 11. 423-426 (1998)

K. Tsuru、S. Hayakawa、C. Ohtsuki 和 A. Osaka：“聚合物基材上涂覆的有机改性陶瓷膜的生物活性”生物陶瓷。

K. Tsuru他: J. Mater. Sci., Materials in Medicine. 9. 479-484 (1998)

K. Tsuru 等人：J. Mater.，医学材料。 9. 479-484 (1998)

K. Tsuru, S. Hayakawa, C. Ohtsuki and A. Osaka: "Ultrasonic implantation of calcium metasilicate glass particles into PMMA"J. Mater. Sci. Materials in Medicine. 9. 479-484 (1998)

K. Tsuru、S. Hayakawa、C. Ohtsuki 和 A. Osaka：“将偏硅酸钙玻璃颗粒超声植入 PMMA”J。

K. Tsuru他: "Bioactivity and structure of organically modified silicates synthesized by the sol-gel method"Bioceramics. 10. 33-36 (1997)

K. Tsuru 等：“溶胶-凝胶法合成的有机改性硅酸盐的生物活性和结构”生物陶瓷。10. 33-36 (1997)