Study of aging system in Pulpal and periodontal tissue

牙髓及牙周组织老化系统的研究

• 批准号: 09307042
• 负责人: SUDA Hideaki
• 金额: $ 24.77万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09307042/
• 关键词: Pulp; Periodontal Tissue; Aging; 骨粗鬆症; 実験的根尖病変; 細胞寿命; アポトーシス; 骨リモデリング現象; 抗原提示細胞; サイトカイン産生

1. Macrophages produced IL-1α in periapical lesions in young rat, and lesion increased slowly.2. Healing in periapical lesions was delayed with aging.3. Reaction of antigen presenting cells was weakened in quantity with aging in rat dental pulp, but that of macrophages and monocytes were not weakened.4. Inhibition of breakdown system in gingiva was one of the reasons of gingival overgrowth in young rat.5. Jaw muscle activity induced by dental pulp chemical stimulation was changed with aging. Activity of alkaline phosphatase in dental pulp was decreased with aging in human.6. Decrease in proliferative ability and the increase in the SPARC expression with aging are involved in the aging alterations of metabolism in periodontal ligament.7. Cellular motility of periodontal ligament cells decreased with donor age as well as cell proliferation. Since the cells reaching senescene fail to express c-fos, the mechanisms linked to cellular senescence may be a possible underlying mechanism for low migration seen in the older cells.8. Blood flow in dental pulp and gingiva changed with aging.9. Production of IL-17 in chronic inflamed periodontitis was effected by immunological response of individual man, not effected by aging.10. Bone mineral density of the lumbar vertebra and the mandible in periodontally healthy women was higher than that in edentulous women.

1.结论：1.巨噬细胞在幼龄大鼠根尖周病变中产生IL-1α，且病变缓慢增加.根尖周病变愈合随年龄增长而延迟.随着年龄的增长，牙髓中抗原提呈细胞的反应在数量上减弱，而巨噬细胞和单核细胞的反应并不减弱.牙龈组织中降解系统的抑制是幼鼠牙龈增生的原因之一.牙髓化学刺激引起的下颌肌活动随年龄增长而变化。人牙髓中碱性磷酸酶活性随年龄增长而降低.牙周膜增殖能力的降低和牙周膜中TGF β 1表达的增加参与了牙周膜代谢的增龄性改变.牙周膜细胞的运动能力随着年龄的增长和细胞增殖而降低。由于达到衰老的细胞不能表达c-fos，与细胞衰老相关的机制可能是在老年细胞中观察到的低迁移的潜在机制。牙髓和牙龈血流量随年龄增长而变化. IL-17在慢性牙周炎中的产生受个体免疫应答的影响，而不受年龄的影响。牙周健康妇女腰椎和下颌骨骨的骨密度高于无牙妇女。

项目成果

Sakata M: "Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells"J Bone Miner Res.. 14. 1486-1492 (1999)

Sakata M：“人牙间充质细胞和上皮细胞培养物中骨保护素（破骨细胞生成抑制因子）的表达”J Bone Miner Res.. 14. 1486-1492 (1999)

Nagai A: "Interleukin-17 production in adult periodontitis"J Dent Res.. 78. 184 (1999)

Nagai A：“成人牙周炎中白细胞介素 17 的产生”J Dent Res.. 78. 184 (1999)

Nishimura F, Takahashi K, Kurihara M, Takashiba S, Murayama Y: "Periodontal disease as a complication of diabetes mellitus."Ann Periodontol. 3(1). 20-29 (1998)

Nishimura F、Takahashi K、Kurihara M、Takashiba S、Murayama Y：“牙周病是糖尿病的并发症。”Ann periodontol。

Nishimura F.: "Periodontal disease as a complication of diabetes mellitus" Annals of periodontology. (in press).

Nishimura F.：“牙周病是糖尿病的并发症”牙周病学年鉴。

永井淳: "炎症歯周組織における細胞性免疫応答の様態" 日本歯科保存学雑誌. 40・秋季特別. 74-74 (1997)

Jun Nagai：“发炎牙周组织的细胞免疫反应”，《日本保守牙科杂志》40/秋季特刊（1997 年）。