Project II - Modeling meningomyelocele in frog using human alleles and folic acid exposure

项目 II - 使用人类等位基因和叶酸暴露模拟青蛙脑膜脊髓膨出

• 批准号: 10154466
• 负责人: Christopher Robert Kintner
• 金额: $ 32.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-01 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10154466
• 关键词: 22q11; 22q11.2; Address; Alleles; Animals; Benign; Biological Assay; Biological Models; Birth; CISH gene; Candidate Disease Gene; Cells; Chromatin; Chromosome Deletion; Clinical; Collaborations; Complex; Congenital Abnormality; Coupled; DNA Methylation; Data; Development; DiGeorge Syndrome; Disease; Dose; Effectiveness; Embryo; Environmental Risk Factor; Etiology; Event; Experimental Models; Folic Acid; Folic Acid Deficiency; Foundations; Gene Deletion; Gene Expression; Genes; Genetic; Goals; Human; Incidence; Individual; Injections; Knock-out; Lead; Literature; Mammals; Measures; Meningomyelocele; Messenger RNA; Modeling; Molecular; Morphogenesis; Movement; Mus; Mutagenesis; Mutate; Mutation; Nature; Neural Tube Closure; Neural Tube Defects; Neural Tube Development; Neural tube; Pathway interactions; Patients; Pharmacology; Phenotype; Play; Process; Rana; Recurrence; Risk; Risk Factors; Role; Spinal; Study models; Sum; Syndrome; Testing; Tetrapoda; Variant; Work; Xenopus; Xenopus laevis; base; de novo mutation; experimental study; gene environment interaction; gene interaction; genetic risk factor; genetic testing; genetic variant; in vivo; loss of function mutation; mouse model; mutant; next generation sequencing; null mutation; planar cell polarity; programs; transcriptome sequencing; xenopus genome

Abstract – Project II: Modeling meningomyelocele in frog using human alleles and FA exposure Neural tube defects (NTDs) are a relatively common birth defect with a complex etiology and gene-environment interactions (GXEs). Genetic factors play an important role, and these gene variants likely interact with one another in gene-gene interactions (GXGs). A major focus of our Program Project is the assessment of de novo mutations that can be assessed in patients with NTDs, and their modulation by environmental risk factors, such as folic acid (FA) accessibility. This complex etiology has made NTDs an extremely challenging syndrome to predict based on genetic testing and to treat clinically. This proposal is a part of a comprehensive Program Project to investigate the genetic basis of the NTD subtype known as meningomyelocele (MM), localized to the spinal neural tube, and occuring in approximately one in every 2,500 births. Project I in the application uses next generation sequencing to identify de novo gene variants that are associated with human MM patients, and assess for recurrence. Even for recurrent mutations, it is critical to functionally evaluate causality in an in vivo setting. This project exploits Xenopus as a high throughput and high content tetrapod experimental model to assess these variants, taking advantage of the fact that the morphogenetic process of neural tube formation and the underlying molecular pathways involved in neurulation are conserved between Xenopus and mammalian embryos. CrispR mutagenesis in F0 Xenopus embryos will be used to test whether genes that lie within deletions of LCR22C-D in the 22q11.2 interval and that substantially increase risk of MM in humans, cause NTDs as null mutations. Gene variants detected in the planar cell polarity pathway that may increase the risk of MM in humans will be tested using rescue experiments in Xenopus, taking advantage of quantitative assays for measuring planar axis formation. Finally, the assessment of MM gene variants will also exploit recent studies in Xenopus, indicating that folate deficiency also causes NTDs as in mammals. Interactions between genetic risk factors and folate deficiency on the incidence of NTDs will be rapidly assessed using Xenopus, as part of the overall goal to determine whether FA alters gene expression and thus the expressivity of critical gene mutants. In sum, project II will use Xenopus as model to prioritize MM gene variants identified in Project I that can be further pursued in experiments in the mouse embryo in project III. Aim 1. Test genes in the minimal 22q11.2 deletion interval for a role in NTDs using Xenopus. Aim 2. Test human MM alleles for impact on neural tube formation in Xenopus embryos. Aim 3. Test GXE by assessing the impact of FA on MM gene phenotypes in Xenopus.

摘要-项目II：使用人类等位基因和FA暴露建立青蛙脑脊膜膨出模型 神经管缺陷是一种相对常见的出生缺陷，具有复杂的病因和基因环境。 相互作用(GXE)。遗传因素起着重要作用，这些基因变异很可能与一种 另一种是基因-基因相互作用(GXGs)。我们计划项目的一个主要焦点是对从头开始的评估 NTDS患者中可以评估的突变及其受环境风险因素的调节，如 作为叶酸(FA)的可及性。这种复杂的病因使NTDS成为一种极具挑战性的综合征 根据基因检测进行预测，并进行临床治疗。该提案是综合计划的一部分 研究被称为脑膜脊髓膨出(MM)的NTD亚型的遗传基础的项目，定位于 脊髓神经管，大约每2500名新生儿中就有一名。应用程序中的项目I使用Next 世代测序以确定与人类多发性骨髓瘤患者相关的从头基因变异，以及 评估复发情况。即使对于反复发生的突变，从功能上评估体内的因果关系也是至关重要的。 布景。本项目利用非洲爪哇作为高通量和高含量的四足动物实验模型来 评估这些变体，利用神经管形成和形成的形态发生过程 参与神经形成的潜在分子通路在非洲爪哇和哺乳动物之间是保守的。 胚胎。在非洲爪哇F0胚胎中的CRISPR突变将被用来测试是否存在缺失的基因 LCR22C-D在22q11.2间隔内的突变，并显著增加人类多发性骨髓瘤的风险，导致NTDS为空 突变。在平面细胞极性途径中检测到的基因变异可能增加MM的风险 人类将在非洲爪哇使用救援实验进行测试，利用定量分析 测量平面轴的形成。最后，MM基因变异的评估也将利用最近在 这表明叶酸缺乏也会导致NTDS，就像在哺乳动物中一样。遗传风险之间的相互作用 将使用非洲爪哇对NTDS发病率的影响因素和叶酸缺乏进行快速评估，作为 总的目标是确定FA是否改变基因表达，从而改变关键基因突变体的表达能力。 总而言之，项目II将使用非洲爪哇作为模型，以确定项目I中确定的MM基因变体的优先顺序 在项目III的小鼠胚胎实验中进一步进行。 目的1.利用非洲爪哇测试最小22q11.2缺失区间内的基因在NTDS中的作用。 目的2.检测人类MM等位基因对非洲爪哇胚胎神经管形成的影响。 目的3.通过评估FA对非洲爪哇MM基因表型的影响来检测GxE。