Antibiotic Resistance Surveillance in Retail Meat and Seafood Samples from South Dakota and North Dakota.

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The objective of the FDA-NARMS cooperative agreement program (CAP) is to build a network of 30 laboratories distributed throughout the United States to determine the prevalence of antimicrobial resistance among bacteria (Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE, and enterococci) isolated from samples of chicken, ground turkey, ground beef, ground pork, tilapia, salmon and shrimp purchased from grocery stores. Our proposed portion of this CAP grant would be to determine the prevalence of antibiotic resistance among these microbes isolated from retail meat and seafood samples from South Dakota and North Dakota stores. One hundred and twenty one retail meat and seafood samples will be purchased each quarter for five years from each of two sites in both South Dakota and North Dakota. These retail meat and seafood samples will be delivered to the Food Safety Microbiology (FSM) Laboratory in the Animal Disease Research and Diagnostic Laboratory at South Dakota State University. All samples will be tested for the presence of Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE and Enterococci using established food safety analysis protocols. The microbes isolated from these food samples will be shipped to FDA-CVM for analysis of antibiotic resistance profiles. Isolates of Campylobacter, Salmonella, and E. coli will be sequenced onsite and uploaded to the FDA database utilizing existing GenomeTrakr pipelines. These results will be compiled and distributed back to the FSM lab for dissemination to the Cooperative Extension Services, Consumers, and Producers.