Antibiotic Resistance Surveillance in Retail Meat and Seafood Samples from South Dakota and North Dakota.

FDA-NARMS合作协议计划(CAP)的目标是建立一个由30人组成的网络 分布在美国各地的实验室，以确定抗菌剂的流行率 细菌(沙门氏菌、弯曲杆菌、气单胞菌、弧菌、大肠杆菌、CPE和 肠球菌)从鸡肉、火鸡末、牛肉末、猪肉末、罗非鱼、 从杂货店购买的三文鱼和虾。我们提议的CAP赠款部分将 是为了确定从零售中分离的这些微生物中抗生素耐药性的流行率 南达科他州和北达科他州商店的肉类和海鲜样品。120 每季度购买一份肉类和海鲜零售样品，为期五年，每季度购买两份 南达科他州和北达科他州都有。这些零售肉类和海鲜样品将是 提交给食品安全微生物学(FSM)实验室的动物疾病研究和 南达科他州立大学的诊断实验室。将对所有样品进行检测以确定是否存在 沙门氏菌、弯曲杆菌、气单胞菌、弧菌、大肠杆菌、铜绿假单胞菌和肠球菌 食品安全分析方案。从这些食品样本中分离出的微生物将被运往 FDA-CVM用于分析抗生素耐药性图谱。弯曲菌、沙门氏菌和E. 将利用现有的GenomeTrakr对大肠杆菌进行现场测序并上传到FDA数据库 管道。这些结果将被汇编并分发回密克罗尼西亚联邦实验室，以便传播到 合作扩展服务、消费者和生产者。

The objective of the FDA-NARMS cooperative agreement program (CAP) is to build a network of 30 laboratories distributed throughout the United States to determine the prevalence of antimicrobial resistance among bacteria (Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE, and enterococci) isolated from samples of chicken, ground turkey, ground beef, ground pork, tilapia, salmon and shrimp purchased from grocery stores. Our proposed portion of this CAP grant would be to determine the prevalence of antibiotic resistance among these microbes isolated from retail meat and seafood samples from South Dakota and North Dakota stores. One hundred and twenty one retail meat and seafood samples will be purchased each quarter for five years from each of two sites in both South Dakota and North Dakota. These retail meat and seafood samples will be delivered to the Food Safety Microbiology (FSM) Laboratory in the Animal Disease Research and Diagnostic Laboratory at South Dakota State University. All samples will be tested for the presence of Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE and Enterococci using established food safety analysis protocols. The microbes isolated from these food samples will be shipped to FDA-CVM for analysis of antibiotic resistance profiles. Isolates of Campylobacter, Salmonella, and E. coli will be sequenced onsite and uploaded to the FDA database utilizing existing GenomeTrakr pipelines. These results will be compiled and distributed back to the FSM lab for dissemination to the Cooperative Extension Services, Consumers, and Producers.