ShEEP Request for Intracellular and Extracellular Protein Signaling Station (IEPSS)

项目摘要 生物技术胞内和胞外蛋白信号站(IEPSS)自动化 标准的蛋白质印迹和免疫分析，并产生可靠的，可重复性的定量数据 做爱。IEPSS提供了广泛的功能，包括单细胞蛋白质印迹， 单个细胞中蛋白质异构体的鉴定和翻译后修饰，蛋白质的分离 根据大小和电荷，在多个样品中测量四个或更多分析物。因此，IEPSS将提供 VAWNYHS调查人员研究潜在的急性和慢性过程的直接好处 导致生理功能障碍、疾病和健康下降，最终导致 提高退伍军人生活质量的干预措施。这些项目包括： 特罗恩博士的研究调查了衰老过程中的身体表现、身体虚弱和健康寿命，并 目前正在研究高强度间歇训练(HIIT)对骨骼肌性能的好处 上了年纪的老兵。IEPSS将允许评估蛋白质标记和翻译后修饰 与线粒体健康和骨骼肌代谢有关。 Fliesler博士的项目研究了进行性视网膜变性和 与冲击波超压所致多发伤相关的视觉功能障碍及新型药物的影响 作为治疗剂的抗氧化剂，以防止、减少或减缓病变的进展 流程。IEPSS将评估炎症、氧化应激和细胞死亡的生物标志物。 沙利文博士的研究调查了转录后基因沉默试剂的发展，如 作为治疗孤儿视网膜变性和常见老年性黄斑病变的候选核酶 退化。IEPSS将允许评估核酶救援策略并量化目标蛋白 光感受器和视网膜上皮细胞的表达和翻译后修饰。 坎蒂博士的研究调查了心脏骤停和心脏干细胞期间的细胞重塑 中介修复。IEPSS将促进造血亚型的蛋白质组图谱以及 巨噬细胞亚群，也可以研究趋化因子/细胞因子。此外，它还能量化蛋白质。 对可逆性缺血作出反应而产生的变化。 冯博士的研究试图找到更有效的方法来理解停车突变是如何 导致人类多巴胺能神经元的选择性退化和随后的帕金森氏症。这个 IEPSS将允许描述蛋白质表达谱和翻译后修饰。 Russo博士的研究试图确定青霉素结合蛋白(PBP)7/8作为一种新的抗病毒药物的作用 鲍曼不动杆菌的微生物靶标。IEPSS将能够解决PBP 7/8和PBP 7/8分子机制 通过在单个和培养的细胞中分析各种外膜蛋白来开发受体靶向治疗 细胞，识别在有或没有PBP 7/8的情况下的翻译后修饰。 法卡斯博士的研究试图发现预防和/或治疗老年性黄斑的潜在疗法 变性(AMD)和遗传性视网膜营养不良(IRD)。IEPSS将允许对效果进行验证 DNA甲基化改变对相应蛋白质表达的影响，并量化细胞间的可变性 小鼠视网膜中的视网膜色素变性1小亚型。 朗博士的研究试图确定外切体在干细胞介导的心脏中的功能作用 修理。IEPSS将加强这项工作，对少量的初始 采样并提供量化细胞因子面板的能力，以响应Exosome治疗和测量 心肌梗死后干细胞和小鼠心脏组织中蛋白质的表达。

Project Abstract The Bio-Techne Intracellular and Extracellular Protein Signaling Station (IEPSS) automates standard western blotting and immunoassay and produces reliable, reproducible quantitative data in a very short time. The IEPSS provides a wide array of capabilities, including single cell western blotting, identification of protein isoforms and post-translational modifications in a single cell, separation of proteins based on size and charge, measure four or more analytes in multiple samples. Thus, the IEPSS will provide immediate benefits for VAWNYHS investigators studying the underlying acute and chronic processes that result in physiological dysfunction, disease, and health decline, ultimately permitting the development of interventions to improve the quality of life in the Veteran population. These projects include: Dr. Troen’s research investigates physical performance, frailty, and healthspan during aging and is presently studying the benefits of high intensity interval training (HIIT) for skeletal muscle performance in aging veterans. The IEPSS will allow assessment of protein markers and posttranslational modifications associated with mitochondrial health and skeletal muscle metabolism. Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the pathological processes. The IEPSS will afford assessment of biomarkers of inflammation, oxidative stress, and cell death. Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents such as ribozymes as candidate therapeutics for orphan retinal degenerations and common age-related macular degeneration. The IEPSS will permit assessment of ribozyme rescue strategy and quantify target protein expression and post-translational modification in photoreceptor and retinal epithelial cells. Dr. Canty’s study investigates cellular remodeling during sudden cardiac arrest and cardiac stem cell mediated repair. The IEPSS will facilitate a proteomic profile of hematopoietic subtypes as well as macrophage subpopulations, also enable to study the chemokines/cytokines. In addition, it quantifies protein changes that arise in response to reversible ischemia. Dr. Feng’s study seeks to identify more efficient methods to understand how mutations of parkin cause the selective degeneration of human dopaminergic neurons and the ensuing Parkinson’s disease. The IEPSS will allow for characterization of protein expression profiles and posttranslational modifications. Dr. Russo’s research seeks to determine the role of penicillin binding protein (PBP) 7/8 as a novel anti- microbial target in XDR A. baumannii. The IEPSS will enable to resolve molecular mechanism of PBP 7/8 and develop receptor-targeted therapies by profiling various outer membrane proteins in single and cultured cells, identifying post-translational modifications in the presence or absence of PBP 7/8. Dr. Farkas’ research seeks to discover potential therapies to prevent and/or treat age-related macular degeneration (AMD) and inherited retinal dystrophies (IRDs). The IEPSS will enable validation of the effects of DNA methylation changes on corresponding protein expression and quantify the cell-to-cell variability of the retinitis pigmentosa 1 minor isoform in the mouse retina. Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated cardiac repair. The IEPSS will enhance this work by performing multiple immunoassays on small quantities of initial sample and providing the ability to quantify cytokine panels in response to exosome therapy and measuring protein expression in stem cells and in murine cardiac tissue following myocardial infarction.